Pricing & Availability
- Clone
- 104 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Ly-5.2, LCA
- Isotype
- Mouse (SJL) IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Compare all formats See Alexa Fluor® 488 spectral data
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
109815 | 25 µg | $85.00 | ||||
109816 | 100 µg | $195.00 |
CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
Product Details
Technical data sheet
Product Details
- Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- B10.S mouse thymocytes and splenocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC – Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis6 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
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- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
-
The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3.
- Application References
(PubMed link indicates BioLegend citation)
-
- Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
- Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
- Suzuki K, et al. 2000. Immunity 13:691. (IHC)
- Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
- Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
- Pascal V, et al. 2007. J. Immunol. 179:1751.
- Burman AC, et al. 2007. Blood 110:1064.
- Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
- Phan TG, et al. 2007. Nature Immunol. 8:992.
- Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
- Wen T, et al. 2013. PNAS. 110:6067. PubMed
- Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
- Product Citations
-
- Jing Li et al. 2018. Immunity. 48(4):773-786 . PubMed
- Peterson EJ, et al. 2019. Mol Syst Biol. 15:e8584. PubMed
- Sinclair LV et al. 2019. Elife. 8 pii: e44210. PubMed
- Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
- Lever JM, et al. 2019. JCI Insight. 4:e125503. PubMed
- Zhang F, et al. 2019. Front Immunol. 2.077777778. PubMed
- Wang L, et al. 2020. Cells. 0.458333333. PubMed
- Marchingo JM, et al. 2020. eLife. 9:e53725.. PubMed
- Wen T, et al. 2013. Proc Natl Acad Sci U S A. 110:6067. PubMed
- Hirasawa M, et al. 2016. J Biol Chem. 291: 7373-7385. PubMed
- Jonas B, et al. 2016. PLoS One. 11: 0159189. PubMed
- Wang X, et al. 2021. EMBO J. 40:e105926. PubMed
- Akkaya M, et al. 2020. Sci Rep. 10:13630. PubMed
- Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
- Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
- Inverso D, et al. 2021. Developmental Cell. 56(11):1677-1693.e10. PubMed
- RRID
- AB_492869 (BioLegend Cat. No. 109815)
AB_492868 (BioLegend Cat. No. 109816)
Antigen Details
- Structure
- Protein tyrosine phosphatase (PTP) family, 180-240 kD
- Distribution
-
All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice
- Function
- Phosphatase, T and B cell activation
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
-
1. Suzuki K, et al. 2000. Immunity 13:691.
- Gene ID
- 19264 View all products for this Gene ID
- UniProt
- View information about CD45.2 on UniProt.org
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Other Formats
View All CD45.2 Reagents Request Custom Conjugation
Description | Clone | Applications |
---|---|---|
Biotin anti-mouse CD45.2 | 104 | FC |
FITC anti-mouse CD45.2 | 104 | FC |
PE anti-mouse CD45.2 | 104 | FC |
Purified anti-mouse CD45.2 | 104 | FC, Block, IHC-F, IP |
APC anti-mouse CD45.2 | 104 | FC |
Alexa Fluor® 488 anti-mouse CD45.2 | 104 | FC |
Alexa Fluor® 647 anti-mouse CD45.2 | 104 | FC |
Pacific Blue™ anti-mouse CD45.2 | 104 | FC |
Alexa Fluor® 700 anti-mouse CD45.2 | 104 | FC |
APC/Cyanine7 anti-mouse CD45.2 | 104 | FC |
PerCP anti-mouse CD45.2 | 104 | FC |
PerCP/Cyanine5.5 anti-mouse CD45.2 | 104 | FC |
PE/Cyanine7 anti-mouse CD45.2 | 104 | FC |
Brilliant Violet 421™ anti-mouse CD45.2 | 104 | FC |
Brilliant Violet 570™ anti-mouse CD45.2 | 104 | FC |
Brilliant Violet 650™ anti-mouse CD45.2 | 104 | FC |
Brilliant Violet 510™ anti-mouse CD45.2 | 104 | FC |
Brilliant Violet 785™ anti-mouse CD45.2 | 104 | FC |
Brilliant Violet 605™ anti-mouse CD45.2 | 104 | FC |
Purified anti-mouse CD45.2 (Maxpar® Ready) | 104 | FC, CyTOF® |
PE/Dazzle™ 594 anti-mouse CD45.2 | 104 | FC |
Brilliant Violet 711™ anti-mouse CD45.2 | 104 | FC |
Alexa Fluor® 594 anti-mouse CD45.2 | 104 | IHC-F |
APC/Fire™ 750 anti-mouse CD45.2 | 104 | FC |
TotalSeq™-A0157 anti-mouse CD45.2 | 104 | PG |
TotalSeq™-B0157 anti-mouse CD45.2 | 104 | PG |
TotalSeq™-C0157 anti-mouse CD45.2 | 104 | PG |
Brilliant Violet 750™ anti-mouse CD45.2 | 104 | FC |
Spark Blue™ 550 anti-mouse CD45.2 | 104 | FC |
Spark NIR™ 685 anti-mouse CD45.2 | 104 | FC |