biolegend流式抗体-Alexa Fluor® 488 anti-mouse CD45.2 Antibody

Pricing & Availability

Clone

104 (See other available formats)

Regulatory Status

RUO

Other Names

Ly-5.2, LCA

Isotype

Mouse (SJL) IgG2a, κ

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Product Citations

publications

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Compare all formats See Alexa Fluor® 488 spectral data

Technical data sheet

Product Details

Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

B10.S mouse thymocytes and splenocytes

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC – Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

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Excitation Laser

Blue Laser (488 nm)

Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation⁴, in vivo and in vitro blocking of B cell responses^1,2, and immunohistochemical staining of acetone-fixed frozen sections³. 

Application References

(PubMed link indicates BioLegend citation)

1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
6. Pascal V, et al. 2007. J. Immunol. 179:1751.
7. Burman AC, et al. 2007. Blood 110:1064.
8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
9. Phan TG, et al. 2007. Nature Immunol. 8:992.
10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed

Product Citations

1. Jing Li et al. 2018. Immunity. 48(4):773-786 . PubMed
2. Peterson EJ, et al. 2019. Mol Syst Biol. 15:e8584. PubMed
3. Sinclair LV et al. 2019. Elife. 8 pii: e44210. PubMed
4. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
5. Lever JM, et al. 2019. JCI Insight. 4:e125503. PubMed
6. Zhang F, et al. 2019. Front Immunol. 2.077777778. PubMed
7. Wang L, et al. 2020. Cells. 0.458333333. PubMed
8. Marchingo JM, et al. 2020. eLife. 9:e53725.. PubMed
9. Wen T, et al. 2013. Proc Natl Acad Sci U S A. 110:6067. PubMed
10. Hirasawa M, et al. 2016. J Biol Chem. 291: 7373-7385. PubMed
11. Jonas B, et al. 2016. PLoS One. 11: 0159189. PubMed
12. Wang X, et al. 2021. EMBO J. 40:e105926. PubMed
13. Akkaya M, et al. 2020. Sci Rep. 10:13630. PubMed
14. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
15. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
16. Inverso D, et al. 2021. Developmental Cell. 56(11):1677-1693.e10. PubMed

RRID

AB_492869 (BioLegend Cat. No. 109815)
AB_492868 (BioLegend Cat. No. 109816)

Antigen Details

Structure

Protein tyrosine phosphatase (PTP) family, 180-240 kD

Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function

Phosphatase, T and B cell activation

Ligand/Receptor

Galectin-1, CD2, CD3, CD4

Biology Area

Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers

Molecular Family

CD Molecules

Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID

19264 View all products for this Gene ID

UniProt

View information about CD45.2 on UniProt.org

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Other Formats

View All CD45.2 Reagents Request Custom Conjugation