JinPan DNA酶I 来源于毕赤酵母(重组,溶液) CAS : 9003-98-9 D128589

产品名称 DNA酶I 来源于毕赤酵母(重组,溶液) 产品名称 Deoxyribonuclease I from Pichia pastoris (Recombinant,Solution) 别名脱氧核糖核酸酶 I 英文别名 Deoxyribonucleate 5′-oligonucleotido-hydrolase;DNase I CAS编号 9003-98-9 分子式 PubChem CID 597667 MDL号 MFCD00130918 EC号 232-667-0 Enzyme commission (EC) Number I.U.B.: 3.1.21.1 规格或纯度 ≥2 units/μl 生化机理 DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both DNA strands are cleaved at approximately the same site in the presence of Mn2+. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. The pH optimum is found to be between 7 and 8. DNAse I from bovine pancreas consists of four chromatographically distinguishable components, A, B, C, and D, with their molar ratios being 4:1:1 respectively. Only minor amounts of D are found. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS)6 and actin7 are known to inhibit the enzyme activity. 储存温度 -20°C储存 运输条件超低温冰袋运输 品牌