CST HA-Tag (C29F4) Rabbit mAb 3724S 3724T

CST HA-Tag (C29F4) Rabbit mAb 3724S

货号： 3724S
品牌： CST

通过使用含有流感血凝素表位 (YPYDVPDYA) 的合成肽对动物进行免疫接种来产生单克隆抗体。

Flow cytometric analysis of HeLa cells, untransfected (blue) or transfected with HA-FoxO4 (green), using HA-Tag (C29F4) Rabbit mAb #3724.流式细胞术分析HeLa细胞，蓝色为未转染组，绿色为HA-FoxO4 转染组，所用抗体为HA-Tag (C29F4) Rabbit mAb #3724。

Immunohistochemical analysis of paraffin-embedded COS cells, untransfected (right) or HA-Tag transfected (left), using HA-Tag (C29F4) Rabbit mAb.免疫组化分析石蜡包埋的COS细胞，下图为未转染组，上图为HA-Tag转染组，所用抗体为HA-Tag (C29F4) Rabbit mAb。

Western blot analysis of extracts from HeLa cells, untransfected or transfected with either HA-FoxO4 or HA-Akt3, using HA-Tag (C29F4) Rabbit mAb.Weatern blot分析HeLa细胞提取物，未转染组和采用 HA-FoxO4 或 HA-Akt3转染组,所用抗体为HA-Tag (C29F4) Rabbit mAb。

Confocal immunofluorescent analysis of COS cells, transfected with an HA-tagged protein (left) or mock-transfected (right), using HA-Tag (C29F4) Rabbit mAb (green). Actin filaments have been labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).免疫荧光分析COS-7细胞提取物。左图为采用HA标签蛋白转染组，下图为mock转染组，所用抗体为HA-Tag (C29F4) Rabbit （绿色），肌动蛋白丝采用DY-554 phalloidin (红色)标记。蓝色伪彩为荧光DNA染料，信息为DRAQ5 ® #4084 。

293T cells were either untransfected (left panel) or transfected with an HA-tagged human CBP construct (right panel), then treated with Forskolin #3828 (30 µM) . Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10⁶ cells and 10 µl of HA-Tag (C29F4) Rabbit mAb, 10 µl of CBP (D9B6) Rabbit mAb, or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP^® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ALS2 exon 1 primers, SimpleChIP^® Human NR4A3 Promoter Primers #4829, and SimpleChIP^® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.