CST 14793S DYKDDDDK Tag (D6W5B) Rabbit mAb (Binds to same epitope as Sigma’s Anti-FLAG ® M2 Antibody)

Flow cytometric analysis of 293 cells, untransfected (blue) or transfected with DYKDDDDK-Tag (green), using DYKDDDDK Tag (D6W5B) Rabbit mAb. Anti-Rabbit IgG (H+L), F(ab)₂ Fragment (Alexa Fluor^® 488 Conjugate) #4412 was used as a secondary antibody.

Confocal immunofluorescent analysis of 293T cells transfected with a GFP-DYKDDDDK-Tag (green) using DYKDDDDK Tag (D6W5B) Rabbit mAb (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with DYKDDDDK-GFP (N-terminal DDK-Tag; +), GFP-DYKDDDDK (C-terminal DDK-Tag; +), human CASQ1-DYKDDDDK (C-terminal DDK-Tag; +), or human FoxG1-DYKDDDDK (C-terminal DDK-Tag; +) as indicated, using DYKDDDDK Tag (D6W5B) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded HeLa cell pellets, control (left) or DYKDDDDK Tag-transfected (right), using DYKDDDDK Tag (D6W5B) Rabbit mAb.

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10⁶ 293T cells, untransfected (left) or transfected (right) with a DYKDDDDK-tagged SSRP1 construct and 10 µl of DYKDDDDK (D6W5B) Rabbit mAb, 10 µl of SSRP1 (E1Y8D) Rabbit mAb #13421, or 2 µl of Normal Rabbit IgG #2729, using SimpleChIP^® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP^® Human c-Fos Exon 3 Primers #12010 and SimpleChIP^® Human EGR1 Intron 3 Primers #11953. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Immunoprecipitation of FoxG1-DYKDDDDK tag protein from 293T transfected cell extracts using Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900 (lane 2) or DYKDDDDK (D6W5B) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using DYKDDDDK (D6W5B) Rabbit mAb.