产品介绍
Alt-R 定制 CRISPR gRNA 文库可用于所有 CRISPR 核酸酶，包括 Cas9、Cas12a、Cas13、主要编辑酶和其他替代系统。开发这些文库是为了满足对更好的CRISPR筛选解决方案的需求。它们是在 IDT 专有的高保真 RNA 生产平台上合成的化学修饰的向导 RNA，可提供高质量、可靠的快速交付 gRNA 文库。
| 特点 | Options |
|---|---|
| 设计 | Predesigned, custom, user-provided |
| CRISPR systems | Cas9, Cas12a, Cas13, prime editing, and other alternative systems |
| 保证得率 | 0.5 nmol, 2 nmol, 5 nmol, and custom normalized deliverables |
| Cas9 gRNA formats | 2-part crRNA:tracrRNA complex and sgRNA |
| Custom lengths supported | 30–150 nt |
| Chemical modifications | 2’-O-methyl RNA, PS linkages, end-blocking Alt-R modifications |
| Plate types | 96- & 384-well PCR, Deep-well, V-bottom, Echo, custom options available |
| Formulation options | Multi-guide per well; pooled by gene Arrayed (single gRNA/well) Custom formulations upon request |
| QC | Individual ESI/MS |
| Supporting reagents & functional analysis pipeline (optional) | WT Cas9, HiFi Cas9, Cas12a. and Cas12a Ultra Glycerol-free options available in tubes or plates (ideal for robotics) Electroporation Enhancers rhAmpSeq™ CRISPR Analysis System (NGS-based on-/off-target editing analysis) |
Alt-R CRISPR 酶
我们的 Alt-R CRISPR 酶有多种形式可供选择，原液大小高达 50 mg。可根据要求为所有产品提供更大的规格和批次匹配.
| Cas protein | Available versions | 核心优势 |
|---|---|---|
| S.p. Cas9 Nuclease V3 | Wild-type, 50% Glycerol Wild-type, Glycerol-free High Fidelity (HiFi) Wild-type Fluorescent-fusion |
Targeting GC-rich regions Low viscosity for high-throughput applications Reduced off-target activity* Fluorescent label for enrichment (GFP or RFP) |
| A.s. Cas12a (Cpf1) V3 | 野生型 Ultra |
Targeting AT-rich regions Increased on-target activity* |
| L.b. Cas12a (Cpf1) | Ultra | Increased on-target activity and low-temperature tolerance* |