NEBNext Ultra II RNA 第二链合成模块
#E6111L100 次反应
#E6111S20 次反应
应用
– RNA Sample Preparation
– Second Strand Synthesis of cDNA
概述
The NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module has been optimized to generate double-stranded cDNA from first-strand cDNA, as part of the NEBNext non-directional RNA library preparation workflow.
The NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module is Designed for Use with the
Following:
– NEBNext Ultra II RNA First Strand Synthesis Module (NEB #E7771)
– NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546)
– NEBNext Ultra II Ligation Module (NEB #E7595)
– NEBNext Ultra™ II Q5® Master Mix (NEB #M0544)
– NEBNext® Oligos for Illumina® (NEB #E7335, #E7500, #E7710, #E7730, #E6609, #E7600,
#E7535, #E7350)
随酶提供的试剂
NEBNext® Second Strand Synthesis Enzyme Mix
NEBNext® Second Strand Synthesis Reaction Buffer