–  Accurate retention of transcript strand of origin information

–  Utilizes dUTP-based methodology

–  Low input amounts (as low as 10ng purified mRNA or ribosomal-depleted RNA, or 100ng Total RNA)

–  Fast workflow (4.5 – 5 hours), with minimal hands-on time

–  Robust, reliable performance

–  Ultra high fidelity amplification with minimized GC bias

The NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® contains reagents for preparation of strand-specific RNA libraries for Illumina sequencing. Please note that adaptors, primers, rRNA depletion

reagents and poly(A) mRNA isolation reagents are not included in the kit and are available separately.

Ultra Directional RNA Library Preparation for Illumina