Purpose and Application
- Buffer for electrophoresis of DNA
Features
- Sterilized, DNase free
- Suitable for analyzing low molecular weight DNA fragments
- Storable for 1 year at room temperature
琼脂糖凝胶电泳所用缓冲液的类型可由DNA片段的大小和迁移后实验的含量来决定。
如果你想分离大于或等于15,000 bp的大DNA,使用TAE缓冲液。如果你不想提取DNA,或者不关心迁移后1000bp或更小的DNA的恢复率,可以使用TBE缓冲液。
上图显示了EzDNA Ladder应用于使用EzRunTAE或EzRunTBE缓冲液的1.5%琼脂糖凝胶,并使用dive – mini在150V下电泳20分钟的结果。可以看到,TAE缓冲液适用于聚合物分离,TBE缓冲液适用于低分子分离。
Specifications
WSE-7051 EzRun TBE | |
---|---|
Major components | Tris, Boric acid, EDTA, 10x concentrated solution |
Package | 500 mL/bottle |
Preparation | 10x dilute with distilled water |
Applicable amount | For 5L, 220 mL per 1 run (About 22 runs with ATTO Submerge Mini chamber) |
Storage | For 1 year at room temperature (unopened) |
Ordering Information
Code No. | Description | Unit |
---|---|---|
2332392 | WSE-7051 EzRun TBE | 1 pk |