AE-1435 EzApply 2D Kit
Purpose and Application
- Extraction of Proteins from Mammalian cell or tissue cultures
- 2D electrophoresis sample buffer
Features
- Separate extraction allows higher protein concentration per gel, spot detection is easy.
- Reducing reagent, alkylating reagent, washing buffer and stratified solutions are also incorporated into a kit
- Low running cost, while allowing short-time extraction and easy sample preparation
- Combination use with AE-1430 EzApply (sample preparation kit for SDS-PAGE) makes it applicable to SDS-PAGE as well as 2-D electrophoresis
Specifications
| AE-1435 EzApply 2D Kit | |
|---|---|
| Major components | Wash buffer: Tris, NaCl Solution 1: Tris, Ampholine, DTT Solution 2: Urea, Thiourea, Surfactant Alkylated reagent: Ampholine, DTT Stratified solutions: Acrylamide, BPB, Urea |
| Package | Solution/30 mL bottle x 2 (Wash buffer) Solution/20 mL bottle (Solution 1) Solution/10 mL bottle (Solution 2) Solution/1 mL tube (Alkylated reagent) Solution/500 μL tube (Stratified solution) Powder/tube (DTT) |
| Preparation | Appropriated amount of solution is applied to tissues or cells, which were lysed and extracted |
| Applicable amount | Extracted from 100 mg of mammalian tissue 20 runs |
| Storage | 6 months at -20℃ (before dissolved in DTT) 1 week after dissolved in DTT |
Ordering Information
| Code No. | Description | Unit |
|---|---|---|
| 2332335 | AE-1435 EzApply 2D Kit | 1 pk |
目的和应用
从哺乳动物细胞或组织培养物中提取蛋白质
二维电泳样品缓冲液
特性
单独提取可使每个凝胶的蛋白质浓度更高，斑点检测容易。
还原试剂、烷基化试剂、洗涤缓冲液和分层溶液也被纳入一个套件
运行成本低，提取时间短，样品制备容易
与AE-1430 EzApply (SDS-PAGE样品制备试剂盒)结合使用，使其适用于SDS-PAGE和二维电泳