WSE-7010 EzLabel FluoroNeo
Purpose and Application
Visualization of electrophoresis pattern with stain FREE
- Protein sample preparation for SDS-PAGE
- Fluorescent labeling for protein and polypeptide
- Protein electrophoresis (1D, 2D-PAGE)
- Immunoprecipitation, etc.
Features
- Immediate visualization protein bands after electrophoresis
Protein fluorescence is emitted by BlueLED or UV excitation light
[Ex: 330 (UV), 470 nm, Em: 530 nm] - Stain FREE, Saving time, Unnecessary for liquid waste disposal
- Hardly to detect any differences in protein band mobility
- Observable the gel with Blue LED without removing glass plate gel holder
- Observed gel is available for western blotting and protein fluorescence is kept after transfer on membrane
- Observed gel is available for CBB staining and silver staining
- Kit includes RIPA Lysis Buffer (free amine buffer), MW marker
Specifications
WSE-7010 EzLabel FluoroNeo | |
---|---|
Components | Sample buffer (5x): 12 mL Labeling reagent: 10 mg Reducing agent (DTT): 300 mg MW marker: 600 μL RIPA Lysis buffer: 10 mL |
Storage | 1 year at -20℃ |
Applicable amount | 2,000 samples, 20 μL/sample |
Ordering Information
Code No. | Description | Unit |
---|---|---|
2332333 | WSE-7010 EzLabel FluroNeo | 1 pk |
目的和应用
无染电泳图形可视化
用于SDS-PAGE的蛋白质样品制备
蛋白质和多肽荧光标记
蛋白质电泳(1D, 2D-PAGE)
免疫沉淀反应等。
特性
电泳后立即可见蛋白条带
蛋白质荧光是由蓝光或紫外线激发光发出的
[例:330 (UV), 470 nm, Em: 530 nm]
无污渍,节省时间,无需处理液体废物
几乎检测不到任何蛋白带流动性的差异
用蓝色LED可以观察到凝胶,不需要移除玻璃板凝胶夹
观察到的凝胶可用于western blotting,蛋白荧光在膜上转移后保存
观察凝胶可用于CBB染色和银染色
试剂盒包括RIPA裂解缓冲液(游离胺缓冲液)、MW标记