WSE-7422 EzSubcell Fraction
Purpose and Application
- Electrophoresis/ Western blotting/ Immunoprecipitation/ ELISA/ Bioactivity/Protein purification/ Chromatin immunoprecipitation assay/ Enzyme activity/ etc.
Features
- Sample preparation for electrophoresis, immunoprecipitation, ELISA, chromatin immunoprecipitation assay, enzyme activity & bioactivity experiments, etc.
- Simple preparation of organella fractionation in high reproducibility and purity.
- Additionally required material is only cooled centrifuge( to 10,000 x g).
- Process for all fractionation is completed in 2 hours.
- Detergent or non-detergent method is available.
Specifications
| WSE-7422 EzSubcell Fraction | |
|---|---|
| Components | Extraction buffer: HEPES, detergent Protease Inhibitor (100X): Aprotinin, Pepstatin A, Leupeptin |
| Applicable amounts | 50 samples, up to 2 x 10⁷ cell/sample |
| Storage | 1 year |
| Package | Fraction buffer 1: 50 mL Fraction buffer 2: 50 mL RIPA Lysis buffer: 20 mL Detergent mix (50X): 1 mL Protease Inhibitor (100X): 0.7 mL |
Ordering Information
| Code No. | Description | Unit |
|---|---|---|
| 2332338 | WSE-7422 EzSubcell Fraction | 1 pk |
目的和应用
电泳/ Western blotting/免疫沉淀/ ELISA/生物活性/蛋白纯化/染色质免疫沉淀测定/酶活性/等
特性
样品制备电泳，免疫沉淀，ELISA，染色质免疫沉淀，酶活性及生物活性实验等。
细胞器分离制备简单，重复性高，纯度高。
此外，所需材料只需冷却离心机(至10,000 x g)。
所有分馏过程在2小时内完成。
可用洗涤剂或非洗涤剂方法。