抗c-Myc磁珠

抗c-Myc磁珠是亲和颗粒，可使用手动或自动磁选机对细菌或哺乳动物细胞或体外系统中表达的重组c-Myc标记蛋白进行免疫沉淀。|抗c-Myc磁珠的特点：| •特异性-高度特异性的抗c-Myc单克隆抗体（克隆9E10）可实现高产量和高纯度的免疫沉淀。|•方便快捷-产品说明提供了易于遵循的优化方案，可在大约一小时内进行免疫沉淀。 •低的非特异性结合-稳定的预先封闭的珠子和特异性抗体可最大程度地减少c-Myc-tag IP或co-IP实验的脱靶结合。|•多功能-珠子与手动和自动工作流程兼容。磁珠表面涂有抗c-My

Anti-c-Myc Magnetic Beads are affinity particles for immunoprecipitation of recombinant c-Myc-tagged proteins expressed in bacterial or mammalian cells or in vitro systems, using manual or robotic magnetic separators.|Features of Anti-c-Myc Magnetic Beads:|• Specific—highly specific anti-c-Myc monoclonal antibody (clone 9E10) enables high yield and high purity immunoprecipitation.|• Convenient and fast—product instructions provide an easy-to-follow, optimized protocol for immunoprecipitation in approximately one hour.|• Low non-specific binding—stable, pre-blocked beads and specific antibody minimize off-target binding for c-Myc-tag IP or co-IP experiments.|• Versatile—beads are compatible with manual and automated workflows.|The blocked magnetic bead surface is coated with anti-c-Myc antibody, a highly specific mouse IgG1 monoclonal antibody (clone 9E10) that recognizes the c-Myc-epitope tag (EQKLISEEDL) derived from the human c-myc oncogene (p62 c-myc). Anti-c-Myc Magnetic Beads can be used manually with a magnetic stand, as well as with automated platforms.|Product Details:|Anti-c-Myc Magnetic Beads are convenient for the immunoprecipitation (IP) of recombinant c-Myc tagged proteins and the co-immunoprecipitation (Co-IP) of their interacting proteins. The beads are incubated with a cell lysate containing c-Myc tagged protein and the fusion protein is captured. The beads are subsequently washed and then the target proteins are eluted using 0.1M glycine (pH 2.0), 50 mM NaOH, or SDS-PAGE sample buffer.
•Do not centrifuge, dry or freeze Anti-c-Myc Magnetic Beads, as this can cause the beads to aggregate and lose binding activity. To ensure good dispersal of beads for optimal antibody binding, it is important to include 0.025% to 0.1% non-ionic (e.g.,sc-2
Anti-c-Myc Magnetic Beadsis for immunoprecipitation of recombinant c-Myc-tagged proteins