Oil Red O Stain Kit 油红O染色试剂盒
Oil Red O油红O；Lipid Droplet 脂滴；Adipocyte differentiation 脂肪细胞分化；3T3-L1 cells；Mesenchymal stem cells (MSCs)；CAS NO: 1320-06-5；
产品信息
| 产品名称 | 产品编号 | 规格 | |
|
Oil Red O Stain Kit 油红O染色试剂盒 |
MM1003-50ML |
50ml |
|
|
Oil Red O Stain Kit 油红O染色试剂盒 |
MM1003-250ML | 250ml |
产品描述
脂滴（Lipid Droplet）是中性脂（主要有脂酰甘油和酯化胆固醇）的主要贮存场所，是脂肪细胞的主要成分，占据脂肪细胞的大部分空间。正常情况下，除脂肪细胞以外的其他细胞内一般不见或仅能看到微量脂滴。但病理情况下，这些细胞中可能出现脂滴或者脂滴明显增多，通过脂肪染色能将这些脂滴清晰的显现出来。比如，心、肝、肾等实质器官发生脂肪变性，胞浆内出现大小不一的空泡，可用脂肪染色来区分是脂肪变性还是水性变性或糖原贮存等。
本油红O染色试剂盒（Oil Red O Stain Kit）是一款基于油红O的脂质染色试剂盒，染液无毒环保，操作简便，性能稳定，而且显色清晰，颜色鲜亮，对比分明，易于观察。染色后的切片能长期保存且不易褪色。本试剂盒适用于冰冻切片、骨髓涂片和血涂片染色。
油红O染色与成脂水平鉴定（图片示例，来自文献）
应用目的：通过油红O染色来鉴定c9, t11-CLA对3T3-L1脂肪前细胞分化为脂肪细胞上的效应。
操作方法：The differentiation of 3T3-L1 preadipocytes was initiated 2 days after confluence for 3 days in growth medium containing 0.25 µM dexamethasone, 0.5 mM IBMX, and 1 µg/ml insulin. This was followed by 2 days in growth medium containing 1 µg/ml insulin. Thereafter, the cells were cultured in the growth medium for 2 days. c9, t11-CLA (100 µM) was added to the medium from day-3 (time of addition of dexamethasone, IBMX, and insulin) to day-9 (end point of the experiment). The treated cells were fixed in 4% formaldehyde-phosphate buffer and stained with 0.3% Oil Red O dye.
实验图片：
Fig 1. Microscopic observations of Oil Red O staining of 3T3-L1 cells treated with c9, t11-CLA. c9, t11-CLA-treated cells contained more and smaller adipocytes with lipid filling compared to the contol.
文献信息：Sakuma S et al. cis9, trans11-Conjugated Linoleic Acid Differentiates Mouse 3T3-L1 Preadipocytes into Mature Small Adipocytes through Induction of Peroxisome Proliferator-activated Receptor γ. J Clin Biochem Nutr. 2010 Sep;47(2):167-73. PMID: 20838573
| 组分编号 | 组分名称 | 储存 |
产品货号（规格） |
|
| MM1003-50ML | MM1003-250ML | |||
| MM1003-A | Oil O Red Stock Solution 油红O储存液 | 室温 | 50ml | 250ml |
| MM1003-B | Oil O Red Dilution Solution油红O稀释液 | 室温 | 20ml | 100ml |
| MM1003-C | Counterstain 复染液 | 室温 | 50ml | 250ml |
| MM1003-D | Aqueous Mounting Medium 水溶性封固剂 | 室温 | 10ml | 2×20ml |
操作方法
一、 样本处理
1.1 冰冻切片：
1.2 骨髓切片/血涂片：
二、 染色工作液的制备
三、 染色步骤（染缸法）
3.1 将骨髓切片、血涂片或冰冻切片直接放入装油红O染色工作液的染缸中，染色10~15min，用37℃左右预温的蒸馏水水洗5~20s，注意水洗时水流不可太大。
3.2 复染液染色3~5min，水洗约30~60s。
3.3 未待表面水干透即可滴加水性封固剂于玻片表面，进行封片。注意：a）封片前应将水溶性封固剂于60℃温水中加热至液状才可封片；b）封片不可用油溶性封固剂封片，即千万不能用中性树胶封片，请用试剂盒内的水溶性封固剂封片，否则会影响脂类染色结果。
3.4 镜检。染色结果：脂肪被染成鲜红色，细胞核染成深蓝色，其他组织被染成淡蓝色。
注意事项
为了您的安全和健康，请穿实验服并戴一次性手套操作。
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Oil Red O Stain Kit 油红O染色试剂盒
Oil Red O Stain Kit 油红O染色试剂盒
Oil Red O Stain Kit 油红O染色试剂盒