DO Supplement -His/-Leu 微生物培养:酵母菌的质粒筛选通常基于营养缺陷型突变菌株的使用而开展,这些菌株在缺乏某种特定培养基组分(比如氨基酸,嘌呤或嘧啶)的情况下无法生长。当转化含有突变基因的质粒后使得阳性转化克隆能够在缺乏必需营养组分的培养基内生长,正因如此,省却成分培养基添加剂(DO medium supplements)能用来筛选转化的酵母培养物。
DO Supplement -His/-Leu
YPD Medium(YEPD Medium);YPDA Medium;YNB; Minimal SD Base;Dropout (DO) Supplements 省却成分添加剂;
产品名称 | 产品编号 | 规格 | |
DO Supplement -His/-Leu | MS6320-10G | 10g |
酵母菌的质粒筛选通常基于营养缺陷型突变菌株的使用而开展,这些菌株在缺乏某种特定培养基组分(比如氨基酸,嘌呤或嘧啶)的情况下无法生长。当转化含有突变基因的质粒后使得阳性转化克隆能够在缺乏必需营养组分的培养基内生长,正因如此,省却成分培养基添加剂(DO medium supplements)能用来筛选转化的酵母培养物。
本品为缺乏组氨酸(His)/亮氨酸(Leu)的省却成分培养基添加剂,与Minimal SD base联合使用,配制成双组分缺陷的酵母菌选择性培养基,俗称双缺培养基(二缺培养基),用于双质粒酵母转化筛选。可用在外源基因在酵母中的表达、异源基因功能互补、酵母单杂交系统和酵母双杂交系统初始质粒转化免疫共沉淀、Y187和AH109酵母交配实验、接合型二倍体筛选。
酵母单(双杂交)研究相关产品
1、富集培养基(Rich Medium 酵母培养)
货号 | 名称 | 规格 | |
MS6301-350G | YPD Agar Medium YPD琼脂培养基 | 350g | |
MS6302-250G | YPD Medium YPD液体培养基 | 250g | |
MS6303-350G | YPDA Agar Medium YPDA琼脂培养基 | 350g | |
MS6304-250G | YPDA Medium YPDA液体培养基 | 250g |
2、基本培养基(Minimal Medium 酵母培养)
货号 | 名称 | 规格 | |
MS6311-467G | Minimal SD Agar Base SD琼脂基础培养基 | 467g | |
MS6312-267G | Minimal SD Base SD基础培养基 | 267g | |
MS6313-270G | Minimal SD Agar Base Gal/Raf SD琼脂基础培养基(含半乳糖/棉子糖) | 270g | |
MS6314-185G | Minimal SD Base Gal/Raf SD基础培养基(含半乳糖/棉子糖) | 185g |
3、 省却成分氨基酸混合物(DO Supplements 酵母培养)
类型 | 名称 | 货号 | 规格 | |
Single Dropout Supplements (SDO)
单一省却成分添加剂 |
DO Supplement -His | MS6316-10G | 10g | |
DO Supplement -Leu | MS6317-10G | 10g | ||
DO Supplement -Trp | MS6318-10G | 10g | ||
DO Supplement -Ura | MS6319-10G | 10g | ||
Double Dropout Supplements (DDO)
双省却成分添加剂 |
DO Supplement -His/-Leu | MS6320-10G | 10g | |
DO Supplement -His/-Ura | MS6321-10G | 10g | ||
DO Supplement -Leu/-Trp | MS6322-10G | 10g | ||
DO Supplement -Met/-Trp | MS6323-10G | 10g | ||
DO Supplement -Trp/-Ura | MS6324-10G | 10g | ||
DO Supplement -Trp/-His | MS6325-10G | 10g | ||
DO Supplement -Ura/-Leu | MS6326-10G | 10g | ||
Triple Dropout Supplements (TDO)
三省却成分添加剂 |
DO Supplement -His/-Leu/-Trp | MS6331-10G | 10g | |
DO Supplement -His/-Leu/-Ura | MS6332-10G | 10g | ||
DO Supplement -His/-Trp/-Ura | MS6333-10G | 10g | ||
DO Supplement -Leu/-Met/-Trp | MS6334-10G | 10g | ||
DO Supplement -Leu/-Trp/-Ura | MS6335-10G | 10g | ||
Quadruple Dropout Supplements (QDO)
四省却成分添加剂 |
DO Supplement -Ade/-His/-Leu/-Trp | MS6341-10G | 10g | |
DO Supplement -His/-Leu/-Met/-Trp | MS6342-10G | 10g | ||
DO Supplement -His/-Leu/-Trp/-Ura | MS6343-10G | 10g |
四、筛选抗生素(底物)
货号 | 名称 | 规格 | |
MS0002-1ML | Aureobasidin A (AbA)金担子素A(1 mg/ml) | 1ml | |
MF2003-25MG | X-α-Gal 5-溴-4-氯-3-吲哚-α-D-吡喃半乳糖苷 | 25mg |
五、酵母质粒提取试剂盒
货号 | 名称 | 规格 | |
D3376-01 | 酵母质粒抽提试剂盒 Yeast Plasmid Kit(Omega Biotek) | 50次 |
六、酵母转化试剂盒
货号 | 名称 | 规格 | |
MF2501-200T | Yeast Transformation Kit 酵母转化试剂盒 | 200T |
MS6320 DO Supplement -His/-Leu 微生物培养
MS6320 DO Supplement -His/-Leu 微生物培养
MS6320