Pricing & Availability
- Clone
- 10-3.6 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- MHC class II
- Isotype
- Mouse (CWB) IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
C3H/He mouse splenocytes stained with 10-3.6 PE -
BALB/c mouse splenocytes stained with 10-3.6 biotin and detected with Sav-PE
Compare all formats See PE spectral data
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 109908 | 200 µg | $265.00 | ||||
The 10-3.6 antibody reacts with the β chain of the I-Ak MHC class II alloantigen. This class II molecule is expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2k bearing mice and involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 10-3.6 antibody cross-reacts with I-Af,r,s antigens and I-Ag7 of NOD mice; it does not react with other haplotypes (e.g., b, d, p, q).
Product Details
Technical data sheet
Product Details
- Reactivity
- Mouse k haplotype, Cross-reacts with H-2 f,r,s haplotypes
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- C3H mouse splenocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC – Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm) - Application Notes
-
Additional reported applications (for the relevant formats) include immunoprecipitation1,2, protection against autoimmune IDDM3, in vitro blocking of antigen-specific MHC-restricted responses and in vivo inhibition of lymphoma growth4, and immunohistochemical staining5 of acetone-fixed frozen sections.This clone does not cross react with other haplotypes (e.g. b, d, p, q).
- Application References
(PubMed link indicates BioLegend citation)
-
- Landais D, et al. 1986. J. Immunol. 137:3002. (IP)
- Kupinski JM, et al. 1983. J. Immunol. 130:2277. (IP)
- Kappler JW, et al. 1981. J. Exp. Med. 153:1198.
- Alisauskas RM, et al. 1986. Immunopharmacology 12:1.
- Reis e Sousa and Germain 1999. J. Immunol. 162:6652. (IHC)
- Yui MA, et al. 2010. J. Immunol. 185:284. PubMed
- Gaudreau S, et al. 2007. J. Immunol. 179:3638. (FC)
- Busman-Sahay K, et al. 2011. J. Immunol. 186:6710. PubMed.
- Product Citations
-
- Wang H, et al. 2020. Nat Mater. 1.655555556. PubMed
- RRID
- AB_313457 (BioLegend Cat. No. 109908)
Antigen Details
- Structure
- β chain of MHC class II
- Distribution
-
B cell and activated T cells, APCs of H-2b mice
- Function
- Antigen presentation
- Ligand/Receptor
- CD3/TCR, CD4
- Cell Type
- B cells, T cells, Antigen-presenting cells, Tregs
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
-
1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323. - Gene ID
- 14960 View all products for this Gene ID
- UniProt
- View information about I-Ak on UniProt.org
Related Products
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Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All I-Ak Reagents Request Custom Conjugation
| Description | Clone | Applications |
|---|---|---|
| FITC anti-mouse I-Ak (Aβk) | 10-3.6 | FC |
| PE anti-mouse I-Ak (Aβk) | 10-3.6 | FC |
| Purified anti-mouse I-Ak (Aβk) | 10-3.6 | FC, IHC-F, IP |