Pricing & Availability
- Clone
- 10.1 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- VI MA36
- Other Names
- FcγRI, FcR I
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Compare all formats See APC/Fire™ 750 spectral data
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| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 305035 | 25 tests | $115.00 | ||||
| 305036 | 100 tests | $300.00 | ||||
DescriptionCD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcR I. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).
CD64 是一种 72 kD 的单链 I 型糖蛋白，也称为 FcγRI 和 FcR I。CD64 是免疫球蛋白超家族的成员，在单核细胞/巨噬细胞、树突状细胞和活化的粒细胞上表达。 表达可以通过干扰素γ 刺激上调。 CD64 结合 IgG 免疫复合物。 它在抗原捕获、IgG/抗原复合物的吞噬作用和抗体依赖性细胞毒性 (ADCC) 中发挥作用。
Product Details
Technical data sheet
Product Details
- Reactivity
- Human, Baboon, Capuchin Monkey, Chimpanzee, Cynomolgus, Rhesus, Squirrel Monkey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human rheumatoid synovial fluid cells and fibronectin-purified monocytes.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- Preparation
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
- FC – Quality tested
- Recommended Usage
- Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Application Notes
- Clone 10.1 recognizes the EC3 epitope of CD64. While both contain the EC3 domain, in-house testing suggests that clone 10.1 preferentially binds to CD64A (FcγRIA), but not CD64B (FcγRIB). Additional reported applications (for the relevant formats) include: blocking of human IgG3 and murine IgG2a binding to FcγRI2,5,6,11 and immunohistochemical staining of acetone-fixed frozen tissue sections12.
- Application References
- 反应性
人、狒狒、卷尾猴、黑猩猩、食蟹猴、恒河猴、松鼠猴
抗体类型
单克隆
宿主物种
老鼠
免疫原
人类风湿滑液细胞和纤连蛋白纯化的单核细胞。
公式
磷酸盐缓冲溶液，pH 7.2，含有 0.09% 叠氮化钠和 0.2% (w/v) BSA（原产美国）。
准备
浓度
0.2毫克/毫升
储存和处理
抗体溶液应在 2°C 至 8°C 之间未经稀释储存，并避免长时间暴露在光线下。不要冻结。
应用
FC – 质量测试推荐用法
每批这种抗体都经过免疫荧光染色和流式细胞术分析的质量控制测试。对于流式细胞仪染色，建议使用该试剂为 100 µl 染色体积中每百万个细胞 5 µl 或每 100 µl 全血 5 µl。
应用笔记
克隆 10.1 识别 CD64 的 EC3 表位。虽然两者都包含 EC3 结构域，但内部测试表明克隆 10.1 优先结合 CD64A (FcγRIA)，而不是 CD64B (FcγRIB)。其他报告的应用（相关格式）包括：阻断人 IgG3 和鼠 IgG2a 与 FcγRI2、5、6、11 的结合以及丙酮固定的冷冻组织切片的免疫组织化学染色 12。应用参考
(PubMed link indicates BioLegend citation) -
- McMichael A, et al. Eds. 1987. Leucocyte Typing III. Oxford University Press. New York.
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. p. 874.
- Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
- Holl V, et al. 2004. J. Immunol. 173:6274.
- Hober D, et al. 2002. J. Gen. Virol. 83:2169.
- Cho HJ, et al. 2007. Physiol Genomics 149:60.
- van Tits L, et al. 2005. Arterioscler Thromb Vasc Biol. 25:717. PubMed
- Bruhns P, et al. 2008. Blood 113:3716. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Carter DL, et al. 1999. Cytometry 37:41. (FC)
- Dougherty GJ, et al. 1987. Eur. J. Immunol. 17:1453.
- Blom AB, et al. 2003. Arthritis Rheum. 48(4):1002-14. (IHC)
- RRID
- AB_2650833 (BioLegend Cat. No. 305035)
AB_2650834 (BioLegend Cat. No. 305036)
Antigen Details
- Structure
- Ig superfamily, type I glycoprotein, 72 kD
- Distribution
- Monocytes, macrophages, dendritic cells, activated granulocytes
- Function
- Phagocytosis, ADCC
- Ligand/Receptor
- IgG receptor
- Cell Type
- Dendritic cells, Granulocytes, Macrophages, Monocytes
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Fc Receptors
- Antigen References
- 1. Hulett M, et al. 1994. Adv. Immunol. 57:1.
2. van de Winkel J, et al. 1993. Immunol. Today 14:215. - Gene ID
- 2209 View all products for this Gene ID
- UniProt
- View information about CD64 on UniProt.org
Related FAQs
- Is our human Trustain FcX™ (cat# 422302) compatible with anti human CD16, CD32 and CD64 clones 3G8, FUN-2 and 10.1 respectively?
- Yes
Other Formats
View All CD64 Reagents Request Custom Conjugation
| Description | Clone | Applications |
|---|---|---|
| Biotin anti-human CD64 | 10.1 | FC |
| FITC anti-human CD64 | 10.1 | FC |
| PE anti-human CD64 | 10.1 | FC |
| Purified anti-human CD64 | 10.1 | FC, IHC-F, Block |
| Alexa Fluor® 488 anti-human CD64 | 10.1 | FC |
| Alexa Fluor® 647 anti-human CD64 | 10.1 | FC |
| APC anti-human CD64 | 10.1 | FC |
| Pacific Blue™ anti-human CD64 | 10.1 | FC |
| Brilliant Violet 421™ anti-human CD64 | 10.1 | FC |
| PE/Cyanine7 anti-human CD64 | 10.1 | FC |
| PerCP/Cyanine5.5 anti-human CD64 | 10.1 | FC |
| APC/Cyanine7 anti-human CD64 | 10.1 | FC |
| Brilliant Violet 510™ anti-human CD64 | 10.1 | FC |
| Purified anti-human CD64 (Maxpar® Ready) | 10.1 | FC, CyTOF® |
| PE/Dazzle™ 594 anti-human CD64 | 10.1 | FC |
| Brilliant Violet 605™ anti-human CD64 | 10.1 | FC |
| APC/Fire™ 750 anti-human CD64 | 10.1 | FC |
| PE anti-human CD64 | 10.1 | FC |
| PE/Dazzle™ 594 anti-human CD64 | 10.1 | FC |
| FITC anti-human CD64 | 10.1 | FC |
| TotalSeq™-A0162 anti-human CD64 | 10.1 | PG |
| Brilliant Violet 711™ anti-human CD64 | 10.1 | FC |
| Alexa Fluor® 700 anti-human CD64 | 10.1 | FC |
| Brilliant Violet 785™ anti-human CD64 | 10.1 | FC |
| TotalSeq™-C0162 anti-human CD64 | 10.1 | PG |
| Ultra-LEAF™ Purified anti-human CD64 | 10.1 | FC, IHC-F, Block |
| TotalSeq™-B0162 anti-human CD64 | 10.1 | PG |
| TotalSeq™-D0162 anti-human CD64 | 10.1 | PG |
| GMP PE anti-human CD64 | 10.1 | FC |
| GMP FITC anti-human CD64 | 10.1 | FC |