Pricing & Availability
- Clone
- 10.1 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- VI MA36
- Other Names
- FcγRI, FcR I
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Compare all formats See Brilliant Violet 711™ spectral data
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| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 305041 | 25 tests | $195.00 | ||||
| 305042 | 100 tests | $395.00 | ||||
DescriptionCD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcR I. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).
Product Details
Technical data sheet
Product Details
- Reactivity
- Human, Baboon, Capuchin Monkey, Chimpanzee, Cynomolgus, Rhesus, Squirrel Monkey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human rheumatoid synovial fluid cells and fibronectin-purified monocytes.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- Concentration
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
- FC – Quality tested
- Recommended Usage
- Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
- Clone 10.1 recognizes the EC3 epitope of CD64. While both contain the EC3 domain, in-house testing suggests that clone 10.1 preferentially binds to CD64A (FcγRIA), but not CD64B (FcγRIB). Additional reported applications (for the relevant formats) include: blocking of human IgG3 and murine IgG2a binding to FcγRI2,5,6,11 and immunohistochemical staining of acetone-fixed frozen tissue sections12.
- 反应性
人、狒狒、卷尾猴、黑猩猩、食蟹猴、恒河猴、松鼠猴
抗体类型
单克隆
宿主物种
老鼠
免疫原
人类风湿滑液细胞和纤连蛋白纯化的单核细胞。
公式
磷酸盐缓冲溶液，pH 7.2，含有 0.09% 叠氮化钠和 BSA（原产美国）。
准备
浓度
特定批次（请联系技术支持以了解浓度和总 µg 量，如果您有批号，请使用我们的查找工具。）
储存和处理
抗体溶液应在 2°C 至 8°C 之间未经稀释储存，并避免长时间暴露在光线下。不要冻结。
应用
FC – 质量测试推荐用法
每批这种抗体都经过免疫荧光染色和流式细胞术分析的质量控制测试。对于流式细胞仪染色，建议使用该试剂为 100 µl 染色体积中每百万个细胞 5 µl 或每 100 µl 全血 5 µl。
本产品受 Sirigen Inc. 的所有权的约束，并在 Sirigen Inc. 的许可下制造和销售。购买本产品即向买方传达了一项不可转让的权利，仅可将所购买的产品用于研究目的。本产品不得转售或以任何方式合并到其他产品中进行转售。严禁用于治疗或诊断。本产品受美国专利、未决专利申请和国外同等专利保护。
激发激光
紫色激光 (405 nm)
应用笔记
克隆 10.1 识别 CD64 的 EC3 表位。虽然两者都包含 EC3 结构域，但内部测试表明克隆 10.1 优先结合 CD64A (FcγRIA)，而不是 CD64B (FcγRIB)。其他报告的应用（相关格式）包括：阻断人 IgG3 和鼠 IgG2a 与 FcγRI2、5、6、11 的结合以及丙酮固定的冷冻组织切片的免疫组织化学染色 12。 - Application References(PubMed link indicates BioLegend citation)
-
- McMichael A, et al. Eds. 1987. Leucocyte Typing III. Oxford University Press. New York.
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. p. 874.
- Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
- Holl V, et al. 2004. J. Immunol. 173:6274.
- Hober D, et al. 2002. J. Gen. Virol. 83:2169.
- Cho HJ, et al. 2007. Physiol Genomics 149:60.
- van Tits L, et al. 2005. Arterioscler Thromb Vasc Biol. 25:717. PubMed
- Bruhns P, et al. 2008. Blood 113:3716. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Carter DL, et al. 1999. Cytometry 37:41. (FC)
- Dougherty GJ, et al. 1987. Eur. J. Immunol. 17:1453.
- Blom AB, et al. 2003. Arthritis Rheum. 48(4):1002-14. (IHC)
- RRID
- AB_2800777 (BioLegend Cat. No. 305041)
AB_2800778 (BioLegend Cat. No. 305042)
Antigen Details
- Structure
- Ig superfamily, type I glycoprotein, 72 kD
- Distribution
- Monocytes, macrophages, dendritic cells, activated granulocytes
- Function
- Phagocytosis, ADCC
- Ligand/Receptor
- IgG receptor
- Cell Type
- Dendritic cells, Granulocytes, Macrophages, Monocytes
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Fc Receptors
- Antigen References
- 1. Hulett M, et al. 1994. Adv. Immunol. 57:1.
2. van de Winkel J, et al. 1993. Immunol. Today 14:215. - Gene ID
- 2209 View all products for this Gene ID
- UniProt
- View information about CD64 on UniProt.org
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Related FAQs
- Is our human Trustain FcX™ (cat# 422302) compatible with anti human CD16, CD32 and CD64 clones 3G8, FUN-2 and 10.1 respectively?
- Yes
Other Formats
View All CD64 Reagents Request Custom Conjugation
| Description | Clone | Applications |
|---|---|---|
| Biotin anti-human CD64 | 10.1 | FC |
| FITC anti-human CD64 | 10.1 | FC |
| PE anti-human CD64 | 10.1 | FC |
| Purified anti-human CD64 | 10.1 | FC, IHC-F, Block |
| Alexa Fluor® 488 anti-human CD64 | 10.1 | FC |
| Alexa Fluor® 647 anti-human CD64 | 10.1 | FC |
| APC anti-human CD64 | 10.1 | FC |
| Pacific Blue™ anti-human CD64 | 10.1 | FC |
| Brilliant Violet 421™ anti-human CD64 | 10.1 | FC |
| PE/Cyanine7 anti-human CD64 | 10.1 | FC |
| PerCP/Cyanine5.5 anti-human CD64 | 10.1 | FC |
| APC/Cyanine7 anti-human CD64 | 10.1 | FC |
| Brilliant Violet 510™ anti-human CD64 | 10.1 | FC |
| Purified anti-human CD64 (Maxpar® Ready) | 10.1 | FC, CyTOF® |
| PE/Dazzle™ 594 anti-human CD64 | 10.1 | FC |
| Brilliant Violet 605™ anti-human CD64 | 10.1 | FC |
| APC/Fire™ 750 anti-human CD64 | 10.1 | FC |
| PE anti-human CD64 | 10.1 | FC |
| PE/Dazzle™ 594 anti-human CD64 | 10.1 | FC |
| FITC anti-human CD64 | 10.1 | FC |
| TotalSeq™-A0162 anti-human CD64 | 10.1 | PG |
| Brilliant Violet 711™ anti-human CD64 | 10.1 | FC |
| Alexa Fluor® 700 anti-human CD64 | 10.1 | FC |
| Brilliant Violet 785™ anti-human CD64 | 10.1 | FC |
| TotalSeq™-C0162 anti-human CD64 | 10.1 | PG |
| Ultra-LEAF™ Purified anti-human CD64 | 10.1 | FC, IHC-F, Block |
| TotalSeq™-B0162 anti-human CD64 | 10.1 | PG |
| TotalSeq™-D0162 anti-human CD64 | 10.1 | PG |
| GMP PE anti-human CD64 | 10.1 | FC |
| GMP FITC anti-human CD64 | 10.1 | FC |