Dojindo,G6PD 检测试剂盒-WST/500/G256

葡萄糖-6-磷酸脱氢酶 (G6PD) 缺乏是最常见的人类酶缺陷之一。 患有这种疾病的人可能会出现由伯氨喹、抗疟疾药物、羟氯喹*等药物引起的溶血性贫血。 Dojindo 的 G6PD 检测试剂盒-WST 使用水溶性四唑盐 WST-8,可在 460 nm 处产生具有强烈橙色的水溶性甲臜。 全血样本中的 G6PD 活性可以通过颜色强度直观地监测。 该试剂盒实现了一种非常简单和快速的 G6PD 活性筛查方法。

*The kit is for research use only.

*Please click this link regarding Hydroxychloroquine.

Principle and data

1) A. Jalloh, I. S. Tantular, S. Pusarawati, A. P. Kawilarang, H. Kerong, K. Lin, M. U. Ferreira, H. Matsuoka, M. Arai, K. Kita and F. Kawamoto, “Rapid Epidemiologic Assessment of Glucose-6-phosphate Dehydrogenase Deficiency in Malaria-endemic Areas in Southeast Asia Using a Novel Diagnostic kit”, Trop. Med. Int. Health, 2004, 9(5), 615.

2) I. S. Tantular, K. Iwai, K. Lin, S. Basuki, T. Horie, H. H. Htay, H. Matsuoka, H. Marwoto, C. Wongsrichanalai, Y. P. Dachlan, S. Kojima, A. Ishii and F. Kawamoto, “Field Trials of a Rapid Test for G6PD Deficiency in Combination with a Rapid Diagnosis of Malaria”, Trop. Med. Int. Health, 1999, 4, 245.

3) A. Ishii, H. Asahi and S.Kawabata, “Glucose-6-Phosphate Dehydrogenase Deficiency in Solomon Islands”, Jpn. J. Parasitol., 1994, 43, 312.

4) E. Beutler, “A Series of New Screening Procedures for Pyruvate Kinase Deficiency, Glucose-6-Phosphate Dehydrogenase Deficiency, and Glutathione Reductase”, Blood, 1966, 28, 553.

5) E. Beutler and M. Mitchell, “Special Modification of the Fluorescent Screening Method for Glucose-6-Phosphate Dehydrogenase Deficiency”, Blood, 1968, 32, 816.

6) H. Fujii, K. Takahashi and S. Miwa, “A New Simple Screening Method for Glucose 6-Phosphate Dehydrogenase Deficiency”, Acta Haematologica Japonica, 1984, 47, 185.

7) A. Hirono, H. Fujii and S. Miwa, “An Improved Single-step Screening Method for Glucose-6-phosphate Dehydrogenase Deficiency”, Jpn. J. Trop. Med. Hyg., 1998, 26, 1.

8) A. Pujades, M. Lewis, A. M. Salvati, S. Miwa, H. Fujii, R. Zarza, R. Alvarez, E. Rull and J. -L. V. Corrons, “Evaluation of the Blue Formazan Spot Test for Screening Glucose 6 Phosphate Dehydrogenase Deficiency”, Int. J. Hematol., 1999, 69, 234.

Dojindo细胞分析

细胞活力和细胞毒性测定用于药物筛选和化学物质的细胞毒性测试。Dojindo开发了高度水溶性的四唑盐,称为WST。WST-8是高度稳定的WST,用于Cell Counting Kit-8(CCK-8)。由于WST-8甲maz是水溶性的,因此不会形成晶体。因此,不需要诸如MTT测定的增溶过程。此外,CCK-8的检测灵敏度高于其他四唑盐,例如MTT,XTT,MTS或WST-1。

WST检测机制

 

ß-半乳糖苷酶检测试剂

细胞增殖/细胞毒性转染细胞染色细胞内荧光探针细菌染色微生物活力测定干细胞分化SPiDER-ßGal线粒体检测细胞代谢

应用 产品展示
细胞生长检测,药物筛选,比色/荧光检测 细胞计数试剂盒-8
细胞计数试剂盒8 + 96孔有机硅定向剂
细胞计数试剂盒-F
细胞毒性LDH检测试剂盒
-WST 96孔有机硅定向剂
MTT
了解检测机制的差异: 点击这里
细胞周期分析 细胞周期测定溶液深红色
细胞周期测定溶液蓝色

 

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