Dojindo,胺反应性PES/10/A543

DescriptionReferencesS.D.S

Product Description

胺反应性 PES 是一种新型的氨基反应性电子介体。 该介体可以通过共价键与葡萄糖脱氢酶等酶连接,有望应用于下一代生物传感器。


新一代电子介体通过用胺反应性PES修饰酶,与酶表面结合的介体能够将电子从还原的辅因子转移到电极。 该传感系统可用于减少电极的介体泄漏和替代昂贵的金属络合物。

Modification of enzymes using Amine-reactive PES is patent pending.


Electrochemical Measurement of Glucose

The concentration of glucose was measured by modifying enzyme using arPES and this sensing system has the potential to develop continuous monitoring for various target molecules such as glucose.


The Sensing System is Applicable to the Following Enzymes– FAD-dependent glucose dehydrogenase (FAD-GDH)– L-Lactate oxidase (Lox)– Fructosyl amino acid oxidase (FAOx)– Cholesterol oxidase (ChOx)– Pyranose oxidase (PyOx)


Procedure for PES-modification

Easy procedure: Just mix enzyme and amine-reactive PES

M. Hatada, N. Loew, Y. Takahashi, J. Okuda-Shimazaki, W. Tsugawa, A. Mulchandani, K. Sode. ” Development of a glucose sensor employing quick and easy modification method with mediator for altering electron acceptor preference.” Bioelectrochemistry, 2018, 121, 185.

Dojindo细胞分析

细胞活力和细胞毒性测定用于药物筛选和化学物质的细胞毒性测试。Dojindo开发了高度水溶性的四唑盐,称为WST。WST-8是高度稳定的WST,用于Cell Counting Kit-8(CCK-8)。由于WST-8甲maz是水溶性的,因此不会形成晶体。因此,不需要诸如MTT测定的增溶过程。此外,CCK-8的检测灵敏度高于其他四唑盐,例如MTT,XTT,MTS或WST-1。

WST检测机制

 

ß-半乳糖苷酶检测试剂

细胞增殖/细胞毒性转染细胞染色细胞内荧光探针细菌染色微生物活力测定干细胞分化SPiDER-ßGal线粒体检测细胞代谢

应用 产品展示
细胞生长检测,药物筛选,比色/荧光检测 细胞计数试剂盒-8
细胞计数试剂盒8 + 96孔有机硅定向剂
细胞计数试剂盒-F
细胞毒性LDH检测试剂盒
-WST 96孔有机硅定向剂
MTT
了解检测机制的差异: 点击这里
细胞周期分析 细胞周期测定溶液深红色
细胞周期测定溶液蓝色

 

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