Mechanism of Thiol Modification with Protein-SHifter:
Fig. 2 Maleimide group of the Protein-SHifter binds to a free thiol group of a protein. The conjugate consists of a unique molecular weight that clearly visualized in electrophoresis.
Visualization of Redox State of Thiol Residues in a Protein:
Fig. 3 One molecule of Protein-SHifter binds to one molecule of free-thiol in protein. The number of the conjugation is directly proportional to the number of free thiol as clear band-shift.
Comparison Data:Linearity of mobility shifts derived from Protein SHifter comparing with PEG-Maleimide (PEG-Mal)
*Conjugation with Thioredoxin
Clear band-shift observed from Protein SHIfter comparing with PEG-Mal.
Detection of Redox State of Thiols residues in GAPDH
15% SDS-polyacrylamidegel
1. Satoshi Hara,Tatsuya Nojima,Kohji Seio, Masasuke Yoshida,Toru Hisabori.”DNA-maleimide: An improved maleimide compound for electrophoresis-based titration of reactive thiols in a specificprotein”Biochimical et Biophysical Acta,2013,1830(4) 3077.2. Satoshi Hara,Yuki Tatenaka, Yuya Ohuchi, Toru Hisabori,”Direct determination of the redox status of cysteine residues in proteins in vivo”. Biochimical and Biophysical Research Communications.2014,In Press.
Dojindo细胞分析
细胞活力和细胞毒性测定用于药物筛选和化学物质的细胞毒性测试。Dojindo开发了高度水溶性的四唑盐，称为WST。WST-8是高度稳定的WST，用于Cell Counting Kit-8（CCK-8）。由于WST-8甲maz是水溶性的，因此不会形成晶体。因此，不需要诸如MTT测定的增溶过程。此外，CCK-8的检测灵敏度高于其他四唑盐，例如MTT，XTT，MTS或WST-1。
WST检测机制
ß-半乳糖苷酶检测试剂
细胞增殖/细胞毒性转染细胞染色细胞内荧光探针细菌染色微生物活力测定干细胞分化SPiDER-ßGal线粒体检测细胞代谢
| 应用 | 产品展示 |
| 细胞生长检测，药物筛选，比色/荧光检测 | 细胞计数试剂盒-8 细胞计数试剂盒8 + 96孔有机硅定向剂 细胞计数试剂盒-F 细胞毒性LDH检测试剂盒 -WST 96孔有机硅定向剂 MTT |
| 了解检测机制的差异： | 点击这里 |
| 细胞周期分析 | 细胞周期测定溶液深红色 细胞周期测定溶液蓝色 |