Pricing & Availability
- Clone
- 10.1 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- VI MA36
- Other Names
- FcγRI, FcR I
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
305023 | 25 tests | $125.00 | ||||
305024 | 100 tests | $305.00 |
CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcR I. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).
Product Details
Technical data sheet
Product Details
- Reactivity
- Human, Baboon, Capuchin Monkey, Chimpanzee, Cynomolgus, Rhesus, Squirrel Monkey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human rheumatoid synovial fluid cells and fibronectin-purified monocytes.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC – Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
-
Clone 10.1 recognizes the EC3 epitope of CD64. While both contain the EC3 domain, in-house testing suggests that clone 10.1 preferentially binds to CD64A (FcγRIA), but not CD64B (FcγRIB). Additional reported applications (for the relevant formats) include: blocking of human IgG3 and murine IgG2a binding to FcγRI2,5,6,11 and immunohistochemical staining of acetone-fixed frozen tissue sections12.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation)
-
- McMichael A, et al. Eds. 1987. Leucocyte Typing III. Oxford University Press. New York.
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. p. 874.
- Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
- Holl V, et al. 2004. J. Immunol. 173:6274.
- Hober D, et al. 2002. J. Gen. Virol. 83:2169.
- Cho HJ, et al. 2007. Physiol Genomics 149:60.
- van Tits L, et al. 2005. Arterioscler Thromb Vasc Biol. 25:717. PubMed
- Bruhns P, et al. 2008. Blood 113:3716. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Carter DL, et al. 1999. Cytometry 37:41. (FC)
- Dougherty GJ, et al. 1987. Eur. J. Immunol. 17:1453.
- Blom AB, et al. 2003. Arthritis Rheum. 48(4):1002-14. (IHC)
- Product Citations
-
- Zimmerman MG, et al. 2018. Cell Host Microbe. 24:731. PubMed
- Llewellyn D, et al. 2015. Sci Rep. 5: 14081. PubMed
- Palamides P, et al. 2016. Dis Model Mech. 9: 985 – 997. PubMed
- RRID
- AB_2561585 (BioLegend Cat. No. 305023)
AB_2561586 (BioLegend Cat. No. 305024)
Antigen Details
- Structure
- Ig superfamily, type I glycoprotein, 72 kD
- Distribution
-
Monocytes, macrophages, dendritic cells, activated granulocytes
- Function
- Phagocytosis, ADCC
- Ligand/Receptor
- IgG receptor
- Cell Type
- Dendritic cells, Granulocytes, Macrophages, Monocytes
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Fc Receptors
- Antigen References
-
1. Hulett M, et al. 1994. Adv. Immunol. 57:1.
2. van de Winkel J, et al. 1993. Immunol. Today 14:215. - Gene ID
- 2209 View all products for this Gene ID
- UniProt
- View information about CD64 on UniProt.org
Related Products
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Related FAQs
- Is our human Trustain FcX™ (cat# 422302) compatible with anti human CD16, CD32 and CD64 clones 3G8, FUN-2 and 10.1 respectively?
-
Yes
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
-
PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
Other Formats
View All CD64 Reagents Request Custom Conjugation
Description | Clone | Applications |
---|---|---|
Biotin anti-human CD64 | 10.1 | FC |
FITC anti-human CD64 | 10.1 | FC |
PE anti-human CD64 | 10.1 | FC |
Purified anti-human CD64 | 10.1 | FC, IHC-F, Block |
Alexa Fluor® 488 anti-human CD64 | 10.1 | FC |
Alexa Fluor® 647 anti-human CD64 | 10.1 | FC |
APC anti-human CD64 | 10.1 | FC |
Pacific Blue™ anti-human CD64 | 10.1 | FC |
Brilliant Violet 421™ anti-human CD64 | 10.1 | FC |
PE/Cyanine7 anti-human CD64 | 10.1 | FC |
PerCP/Cyanine5.5 anti-human CD64 | 10.1 | FC |
APC/Cyanine7 anti-human CD64 | 10.1 | FC |
Brilliant Violet 510™ anti-human CD64 | 10.1 | FC |
Purified anti-human CD64 (Maxpar® Ready) | 10.1 | FC, CyTOF® |
PE/Dazzle™ 594 anti-human CD64 | 10.1 | FC |
Brilliant Violet 605™ anti-human CD64 | 10.1 | FC |
APC/Fire™ 750 anti-human CD64 | 10.1 | FC |
PE anti-human CD64 | 10.1 | FC |
PE/Dazzle™ 594 anti-human CD64 | 10.1 | FC |
FITC anti-human CD64 | 10.1 | FC |
TotalSeq™-A0162 anti-human CD64 | 10.1 | PG |
Brilliant Violet 711™ anti-human CD64 | 10.1 | FC |
Alexa Fluor® 700 anti-human CD64 | 10.1 | FC |
Brilliant Violet 785™ anti-human CD64 | 10.1 | FC |
TotalSeq™-C0162 anti-human CD64 | 10.1 | PG |
Ultra-LEAF™ Purified anti-human CD64 | 10.1 | FC, IHC-F, Block |
TotalSeq™-B0162 anti-human CD64 | 10.1 | PG |
TotalSeq™-D0162 anti-human CD64 | 10.1 | PG |
GMP PE anti-human CD64 | 10.1 | FC |
GMP FITC anti-human CD64 | 10.1 | FC |