Fig. 1 Cell staining mechanism
*caution* Please tap the tube before opening, and open it with care. The content may have relocated from the bottom of the tube during the shipping.
*caution*Please tap the tube before opening, and open it with care. The content may have relocated from the bottom of the tube during the shipping.
Staining Procedure1.Prepare 1 mM Calcein-AM solution with DMSO and dilute to prepare 1-50 μM Calcein-AM solution with PBS.a) 2.Add Calcein-AM solution with 1/10 of the volume of cell culture medium to the cell culture.b) 3.Incubate the cell at 37ºC for 15-30 min. 4.Wash cells twice with PBS or an appropriate buffer. 5.Observe the cells under a fluorescence microscope with 490 nm excitation and 515 nm emission filters.
a) If the Calcein-AM has difficulty loading into cells, use a detergent such as Pluronic F127.b) Or you may replace the culture medium with 1/10 concentration of Calcein-AM buffer solution.
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