biolegend流式抗体-Alexa Fluor® 488 anti-Bcl-2 Antibody

Pricing & Availability

Clone
100 (See other available formats)
Regulatory Status
RUO
Other Names
B-Cell CLL/Lymphoma 2 (Bcl-2), Protein Phosphatase 1, Regulatory Subunit 50
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
Alexa Fluor® 488 anti-Bcl-2 Antibody
Human peripheral blood lymphocytes were treated with Fixation Buffer (Cat# 420801) and Permeabilization Wash Buffer (Cat# 421002), then stained with Bcl-2 (clone 100) Alexa Fluor® 488 (filled histogram) or mouse IgG1, κ Alexa Fluor® 488 isotype control (open histogram).
  • Alexa Fluor® 488 anti-Bcl-2 Antibody
    Human peripheral blood lymphocytes were treated with Fixation Buffer (Cat# 420801) and Permeabilization Wash Buffer (Cat# 421002), then stained with Bcl-2 (clone 100) Alexa Fluor® 488 (filled histogram) or mouse IgG1, κ Alexa Fluor® 488 isotype control (open histogram).

Compare all formats See Alexa Fluor® 488 spectral data

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658703 25 tests $135.00

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658704 100 tests $315.00

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Description

Bcl-2 is a conserved anti-apoptotic protein that plays important roles in normal immunity. It forms homodimers or heterodimers with other Bcl-2 family members. This protein regulates apoptosis through controlling mitochondrial fusion and fission. Phosphorylation of Bcl-2 has been shown to enhance activity to allow response to extracellular growth-factor-mediated signals. Bcl-2 is also regulated by IRES, miR-15a, miR-16-1 and RNA-BP nucleolin. Chromosome 18q21.3 translocation and overexpression of Bcl-2 is frequently observed in follicular lymphomas and some diffuse large B-cell lymphomas.

Product Details

Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide of amino acids 41-54 of human bcl-2 oncoprotein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC – Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

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Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)

  1. Pezzella F, et al. 1993. New Eng. J. Med. 329:690. (IHC)
  2. Pezzella F, et al. 1990. Am. J. Pathol. 137:225. (IHC)
Product Citations
  1. Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed
RRID
AB_2563151 (BioLegend Cat. No. 658703)
AB_2563152 (BioLegend Cat. No. 658704)

Antigen Details

Structure
Distribution
Outer mitochondrial membrane, nuclear membrane, endoplasmic reticulum membrane.
Function
Regulates apoptosis through controlling mitochondrial fusion and fission.
Interaction
BAX, BAD, BAK, Bcl-X(L),EI24,APAF1, BBC3, BCL2L1, BNIPL, MRPL41, TP53BP2, FKBP8 BAG1, RAF1, EGLN3, G0S2, and BOP.
Cell Type
B cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics, Immunology, Mitochondrial Function, Neuroscience, Signal Transduction, Transcription Factors, Ubiquitin/Protein Degradation
Antigen References

1. Lin P, et al. 2013. Curr. Hematol. Malig. Rep. 8:243.
2. Tomita N. 2011. J. Clin. Exp. Hematop. 51:7.
3. Kelly PN, et al. 2011. Cell Death Differ. 18:1414.
4. Willimott S, et al. 2010. Biochem. Soc. Trans. 38:1571.
5. Rolland SG, et al. 2010. Curr. Opin. Cell Biol. 22:852.

Gene ID
596 View all products for this Gene ID
UniProt
View information about Bcl-2 on UniProt.org

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Description Clone Applications

Related FAQs

There are no FAQs for this product.

Other Formats

View All Bcl-2 Reagents Request Custom Conjugation

Description Clone Applications
Purified anti-Bcl-2 100 WB, IHC-F, IHC-P
Alexa Fluor® 647 anti-Bcl-2 100 ICFC
Alexa Fluor® 488 anti-Bcl-2 100 ICFC
PE anti-Bcl-2 100 ICFC
Brilliant Violet 421™ anti-Bcl-2 100 ICFC

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