Pricing & Availability
- Clone
- 104 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Ly-5.2, LCA
- Isotype
- Mouse (SJL) IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![104_Alx700_020608 Alexa Fluor® 700 anti-mouse CD45.2 Antibody](http://www.hamptonresearch.cn/wp-content/uploads/2022/03/20220306_62246dd208f68.jpg)
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C57BL/6 mouse splenocytes stained with 104 Alexa Fluor® 700
Compare all formats See Alexa Fluor® 700 spectral data
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
109821 | 25 µg | $85.00 | ||||
109822 | 100 µg | $195.00 |
CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
Product Details
Technical data sheet
Product Details
- Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- B10.S mouse thymocytes and splenocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC – Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis
* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer’s capability of exciting and detecting the fluorochrome.
View full statement regarding label licenses
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
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The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3.
- Application References
(PubMed link indicates BioLegend citation)
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- Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
- Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
- Suzuki K, et al. 2000. Immunity 13:691. (IHC)
- Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
- Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
- Pascal V, et al. 2007. J. Immunol. 179:1751.
- Burman AC, et al. 2007. Blood 110:1064.
- Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
- Phan TG, et al. 2007. Nature Immunol. 8:992.
- Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
- Wen T, et al. 2013. PNAS. 110:6067. PubMed
- Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
- Product Citations
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- Zaid A,et al. 2017. J Immunol. 10.4049/jimmunol.1700571. PubMed
- Böttcher JP, et al. 2018. Cell. 172:1022. PubMed
- Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed
- Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed
- Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
- Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
- Booth CAG, et al. 2018. Cancer Cell. 33:274. PubMed
- Adams NM et al. 2018. Immunity. 48(6):1172-1182 . PubMed
- Geary CD et al. 2018. Cell reports. 24(8):1949-1957 . PubMed
- Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
- Bradley CP et al. 2017. Cell host & microbe. 22(5):697-704 . PubMed
- Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
- Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed
- Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
- Kubli SP, et al. 2019. Nat Commun. 10:2678. PubMed
- Contreras NA, et al. 2019. PLoS Pathog. 15:e1007890. PubMed
- Page N, et al. 2018. Immunity. 48:937. PubMed
- Louveau A, et al. 2018. Nat Neurosci. 21:1380. PubMed
- He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
- Kim TJ, et al. 2019. Nat Commun. 10:3258. PubMed
- Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
- Sparber F, et al. 2019. Cell Host Microbe. 25:389. PubMed
- Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
- Nabet BY et al. 2017. Cell. 170(2):352-366 . PubMed
- Ersching J et al. 2017. Immunity. 46(6):1045-1058 . PubMed
- Benci JL et al. 2016. Cell. 167(6):1540-1554 . PubMed
- Lagares D, et al. 2017. Sci Transl Med. 9:eaal3765. PubMed
- Urata S, et al. 2018. PLoS Pathog. 14:e1007172. PubMed
- Denny JE, et al. 2019. Sci Rep. 2.786111111. PubMed
- Di Genua C, et al. 2019. Haematologica. 104:2215. PubMed
- Patel S, et al. 2019. Viruses. 0.877083333. PubMed
- Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
- Ciecko AE, et al. 2019. Cell Rep. 29:3073. PubMed
- Mesin L, et al. 2020. Cell. 180(1):92-106.e11.. PubMed
- Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
- Fallet B, et al. 2020. Cell Rep. 30:1013. PubMed
- Horkova V, et al. 2020. Cell Reports. 30(5):1504-1514.e7.. PubMed
- Yoshimi A, et al. 2019. Nature. 574:273. PubMed
- Di Genua C, et al. 2020. Cancer Cell. 37(5):690-704.e8.. PubMed
- Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
- Chandran S, et al. 2020. Front Immunol. 11:787. PubMed
- Kawabe T, et al. 2020. Nat Commun. 2.795833333. PubMed
- Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed
- Loo CS, et al. 2020. Immunity. 53:143. PubMed
- Leruste A, et al. 2020. Cancer Cell. 36(6):597-612.e8.. PubMed
- Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
- He J, et al. 2020. Cell Reports. 29(9):2718-2730.e6.. PubMed
- Zenke S, et al. 2020. Immunity. 52(2):313-327. PubMed
- Wang Y, et al. 2020. eLife. 9:e57438.. PubMed
- Diaz–Salazar C, et al. 2020. Cell Rep. 32:108186. PubMed
- Kang S, et al. 2011. J Exp Med. 208:747. PubMed
- Semmrich M, et al. 2011. Mucosal Immunol. 0.3125. PubMed
- Zinselmeyer B, et al. 2013. J Exp Med. 210:757. PubMed
- Basu S, et al. 2013. J Immunol Methods. 396:163. PubMed
- Markey K, et al. 2014. J Immunol. 192:5426. PubMed
- DeFranco D 2014. Proc Natl Acad Sci U S A. 111:3224. PubMed
- Nadrah K, et al. 2015. PLoS One. 10: 0136061. PubMed
- Kretschmer B, et al. 2015. Immunobiology. 220: 964-975. PubMed
- Luck H, et al. 2015. Cell Metab. 21 527 . PubMed
- XS R, et al. 2015. Diabetes. 64 90. PubMed
- Amos J, et al. 2015. J Virol. 89: 9485-9498. PubMed
- X X, et al. 2016. J Immunol . 197: 1683-1691. PubMed
- Kurowska-Stolarska M, et al. 2016. J Allergy Clin Immunol. S0091-6749(16)31132-0. PubMed
- Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
- Panciera T, et al. 2016. Cell Stem Cell. 19:725-737. PubMed
- Rao S, et al. 2017. Cell. 168(3):503-516.e12. PubMed
- Wiedemann GM, et al. 2020. Cell Rep. 33:108498. PubMed
- Amor C, et al. 2020. Nature. 583:127. PubMed
- Hildreth AD, et al. 2020. STAR Protoc. 1:100113. PubMed
- Schilke RM, et al. 2020. Immunohorizons. 0.624305556. PubMed
- Iwanami N, et al. 2020. iScience. 23:101260. PubMed
- Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
- Plumlee CR, et al. 2020. Cell Host Microbe. 29(1):68-82.e5. PubMed
- Guendel F, et al. 2020. Immunity. 53(5):1015-1032.e8. PubMed
- Rustenhoven J, et al. 2021. Cell. 184(4):1000-1016.e27. PubMed
- Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed
- Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
- Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
- Loo Yau H, et al. 2021. Molecular Cell. 81(7):1469-1483.e8. PubMed
- Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
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- Sheppard S, et al. 2021. Cell Reports. 35(9):109210. PubMed
- RRID
- AB_493730 (BioLegend Cat. No. 109821)
AB_493731 (BioLegend Cat. No. 109822)
Antigen Details
- Structure
- Protein tyrosine phosphatase (PTP) family, 180-240 kD
- Distribution
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All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice
- Function
- Phosphatase, T and B cell activation
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
-
1. Suzuki K, et al. 2000. Immunity 13:691.
- Gene ID
- 19264 View all products for this Gene ID
- UniProt
- View information about CD45.2 on UniProt.org
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Other Formats
View All CD45.2 Reagents Request Custom Conjugation
Description | Clone | Applications |
---|---|---|
Biotin anti-mouse CD45.2 | 104 | FC |
FITC anti-mouse CD45.2 | 104 | FC |
PE anti-mouse CD45.2 | 104 | FC |
Purified anti-mouse CD45.2 | 104 | FC, Block, IHC-F, IP |
APC anti-mouse CD45.2 | 104 | FC |
Alexa Fluor® 488 anti-mouse CD45.2 | 104 | FC |
Alexa Fluor® 647 anti-mouse CD45.2 | 104 | FC |
Pacific Blue™ anti-mouse CD45.2 | 104 | FC |
Alexa Fluor® 700 anti-mouse CD45.2 | 104 | FC |
APC/Cyanine7 anti-mouse CD45.2 | 104 | FC |
PerCP anti-mouse CD45.2 | 104 | FC |
PerCP/Cyanine5.5 anti-mouse CD45.2 | 104 | FC |
PE/Cyanine7 anti-mouse CD45.2 | 104 | FC |
Brilliant Violet 421™ anti-mouse CD45.2 | 104 | FC |
Brilliant Violet 570™ anti-mouse CD45.2 | 104 | FC |
Brilliant Violet 650™ anti-mouse CD45.2 | 104 | FC |
Brilliant Violet 510™ anti-mouse CD45.2 | 104 | FC |
Brilliant Violet 785™ anti-mouse CD45.2 | 104 | FC |
Brilliant Violet 605™ anti-mouse CD45.2 | 104 | FC |
Purified anti-mouse CD45.2 (Maxpar® Ready) | 104 | FC, CyTOF® |
PE/Dazzle™ 594 anti-mouse CD45.2 | 104 | FC |
Brilliant Violet 711™ anti-mouse CD45.2 | 104 | FC |
Alexa Fluor® 594 anti-mouse CD45.2 | 104 | IHC-F |
APC/Fire™ 750 anti-mouse CD45.2 | 104 | FC |
TotalSeq™-A0157 anti-mouse CD45.2 | 104 | PG |
TotalSeq™-B0157 anti-mouse CD45.2 | 104 | PG |
TotalSeq™-C0157 anti-mouse CD45.2 | 104 | PG |
Brilliant Violet 750™ anti-mouse CD45.2 | 104 | FC |
Spark Blue™ 550 anti-mouse CD45.2 | 104 | FC |
Spark NIR™ 685 anti-mouse CD45.2 | 104 | FC |