biolegend流式抗体-APC/Cyanine7 anti-mouse CD45.2 Antibody

Pricing & Availability

Clone

104 (See other available formats)

Regulatory Status

RUO

Other Names

Ly-5.2, LCA

Isotype

Mouse (SJL) IgG2a, κ

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Product Citations

publications

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Compare all formats See APC/Cyanine7 spectral data

Technical data sheet

Product Details

Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

B10.S mouse thymocytes and splenocytes

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC – Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Red Laser (633 nm)

Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation⁴, in vivo and in vitro blocking of B cell responses^1,2, and immunohistochemical staining of acetone-fixed frozen sections³. 

Additional Product Notes

BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact if you have any questions.

Application References

(PubMed link indicates BioLegend citation)

1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
6. Pascal V, et al. 2007. J. Immunol. 179:1751.
7. Burman AC, et al. 2007. Blood 110:1064.
8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
9. Phan TG, et al. 2007. Nature Immunol. 8:992.
10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed

Product Citations

1. Ma W, et al. 2017. Cell Death & Disease. 10.1038/cddis.2017.47. PubMed
2. Thiriot A et al. 2017. BMC biology. 15(1):45 . PubMed
3. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
4. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
5. Cong J et al. 2018. Cell metabolism. 28(2):243-255 . PubMed
6. Fan MY et al. 2018. Cell reports. 25(5):1204-1213 . PubMed
7. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
8. Xu M et al. 2018. Immunity. 48(4):787-798 . PubMed
9. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
10. Glodde N et al. 2017. Immunity. 47(4):789-802 . PubMed
11. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
12. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
13. Bitschar K, et al. 2019. Nat Commun. 10:2730. PubMed
14. Li C, et al. 2018. Cell. 174:285. PubMed
15. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
16. Katagiri T, et al. 2019. Mucosal Immunol. 12:p1104. PubMed
17. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
18. Peloquin GL, et al. 2019. Respir Res. 20:123. PubMed
19. Mintz MA, et al. 2019. Immunity. 51:310. PubMed
20. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
21. Niven J, et al. 2019. Cell Rep. 28:21. PubMed
22. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
23. Codina A, et al. 2019. Cell Syst. 8:136. PubMed
24. Chen Z et al. 2019. Immunity. 51(5):840-855 . PubMed
25. Zhou J, et al. 2019. Immunity. 50:403. PubMed
26. Baryawno N et al. 2019. Cell. 177(7):1915-1932 . PubMed
27. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
28. Oliveira AC et al. 2017. eLife. 6 pii: e30883. PubMed
29. Poh AR, et al. 2017. Cancer Cell. 31:563. PubMed
30. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
31. Ajith A, et al. 2019. FASEB J. 33:5220. PubMed
32. Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed
33. Wang W, et al. 2020. Immunity. 51(6):1102-1118.e7.. PubMed
34. Leruste A, et al. 2020. Cancer Cell. 36(6):597-612.e8.. PubMed
35. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
36. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
37. Béguelin W, et al. 2020. Cancer Cell. 37(5):655-673. PubMed
38. Doo DW, et al. 2020. Ther Adv Med Oncol. 12:1758835920913798. PubMed
39. Gravano D, et al. 2010. PLoS One. 5:e13528. PubMed
40. Kang S, et al. 2011. J Exp Med. 208:747. PubMed
41. Li H, et al. 2011. Blood. 117:697. PubMed
42. Tran I, et al. 2013. J Clin Invest. 123:1590. PubMed
43. Bretz N, et al. 2014. Immunol Lett. 161:140. PubMed
44. White C, et al. 2015. J Immunol. 194:697. PubMed
45. Schaffert S, et al. 2015. J Immunol. 195: 1470-1479. PubMed
46. Siggs O, et al. 2015. Proc Natl Acad Sci U S A. 112: E5706 – E5714. PubMed
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48. Laubreton D, et al. 2020. Viruses. 12:00. PubMed
49. Si Y, et al. 2020. Sci Adv. 6:eaba0995. PubMed
50. Xu C, et al. 2020. Immunity. 53(2):417-428.e4. PubMed
51. Effern M, et al. 2020. Immunity. 53(3):564-580.e9. PubMed
52. Chang D, et al. 2020. Immunity. 53(3):614-626.e4. PubMed
53. Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed
54. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
55. Chen Z, et al. 2021. Cell. 184(5):1262-1280.e22. PubMed
56. Kenswil KJG, et al. 2021. Cell Stem Cell. 28(4):653-670.e11. PubMed
57. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
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RRID

AB_830788 (BioLegend Cat. No. 109823)
AB_830789 (BioLegend Cat. No. 109824)

Antigen Details

Structure

Protein tyrosine phosphatase (PTP) family, 180-240 kD

Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function

Phosphatase, T and B cell activation

Ligand/Receptor

Galectin-1, CD2, CD3, CD4

Biology Area

Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers

Molecular Family

CD Molecules

Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID

19264 View all products for this Gene ID

UniProt

View information about CD45.2 on UniProt.org

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Related FAQs

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Other Formats

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