Pricing & Availability
- Clone
- 104 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Ly-5.2, LCA
- Isotype
- Mouse (SJL) IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![104_AFire750_CD45point2_Antibody_081716_updated APC/Fire™ 750 anti-mouse CD45.2 Antibody](http://www.hamptonresearch.cn/wp-content/uploads/2022/03/20220306_62246d9e9391d.jpg)
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C57BL/6 mouse splenocytes were stained with CD45.2 (clone 104) APC/Fire™ 750 (filled histogram) or mouse IgG2a, κ APC/Fire™ 750 isotype control (open histogram).
Compare all formats See APC/Fire™ 750 spectral data
Cat # | Size | Price | Quantity Check Availability | Save | ||
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109851 | 25 µg | $115.00 | ||||
109852 | 100 µg | $275.00 |
CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
Product Details
Technical data sheet
Product Details
- Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- B10.S mouse thymocytes and splenocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC – Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis
- Application Notes
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The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3.
- Application References
(PubMed link indicates BioLegend citation)
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- Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
- Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
- Suzuki K, et al. 2000. Immunity 13:691. (IHC)
- Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
- Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
- Pascal V, et al. 2007. J. Immunol. 179:1751.
- Burman AC, et al. 2007. Blood 110:1064.
- Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
- Phan TG, et al. 2007. Nature Immunol. 8:992.
- Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
- Wen T, et al. 2013. PNAS. 110:6067. PubMed
- Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
- RRID
- AB_2629722 (BioLegend Cat. No. 109851)
AB_2629723 (BioLegend Cat. No. 109852)
Antigen Details
- Structure
- Protein tyrosine phosphatase (PTP) family, 180-240 kD
- Distribution
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All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice
- Function
- Phosphatase, T and B cell activation
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
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1. Suzuki K, et al. 2000. Immunity 13:691.
- Gene ID
- 19264 View all products for this Gene ID
- UniProt
- View information about CD45.2 on UniProt.org
Related Products
Description | Clone | Applications |
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Related FAQs
There are no FAQs for this product.
Other Formats
View All CD45.2 Reagents Request Custom Conjugation
Description | Clone | Applications |
---|---|---|
Biotin anti-mouse CD45.2 | 104 | FC |
FITC anti-mouse CD45.2 | 104 | FC |
PE anti-mouse CD45.2 | 104 | FC |
Purified anti-mouse CD45.2 | 104 | FC, Block, IHC-F, IP |
APC anti-mouse CD45.2 | 104 | FC |
Alexa Fluor® 488 anti-mouse CD45.2 | 104 | FC |
Alexa Fluor® 647 anti-mouse CD45.2 | 104 | FC |
Pacific Blue™ anti-mouse CD45.2 | 104 | FC |
Alexa Fluor® 700 anti-mouse CD45.2 | 104 | FC |
APC/Cyanine7 anti-mouse CD45.2 | 104 | FC |
PerCP anti-mouse CD45.2 | 104 | FC |
PerCP/Cyanine5.5 anti-mouse CD45.2 | 104 | FC |
PE/Cyanine7 anti-mouse CD45.2 | 104 | FC |
Brilliant Violet 421™ anti-mouse CD45.2 | 104 | FC |
Brilliant Violet 570™ anti-mouse CD45.2 | 104 | FC |
Brilliant Violet 650™ anti-mouse CD45.2 | 104 | FC |
Brilliant Violet 510™ anti-mouse CD45.2 | 104 | FC |
Brilliant Violet 785™ anti-mouse CD45.2 | 104 | FC |
Brilliant Violet 605™ anti-mouse CD45.2 | 104 | FC |
Purified anti-mouse CD45.2 (Maxpar® Ready) | 104 | FC, CyTOF® |
PE/Dazzle™ 594 anti-mouse CD45.2 | 104 | FC |
Brilliant Violet 711™ anti-mouse CD45.2 | 104 | FC |
Alexa Fluor® 594 anti-mouse CD45.2 | 104 | IHC-F |
APC/Fire™ 750 anti-mouse CD45.2 | 104 | FC |
TotalSeq™-A0157 anti-mouse CD45.2 | 104 | PG |
TotalSeq™-B0157 anti-mouse CD45.2 | 104 | PG |
TotalSeq™-C0157 anti-mouse CD45.2 | 104 | PG |
Brilliant Violet 750™ anti-mouse CD45.2 | 104 | FC |
Spark Blue™ 550 anti-mouse CD45.2 | 104 | FC |
Spark NIR™ 685 anti-mouse CD45.2 | 104 | FC |