biolegend流式抗体-Brilliant Violet 421™ anti-mouse CD45.2 Antibody

Pricing & Availability

Clone

104 (See other available formats)

Regulatory Status

RUO

Other Names

Ly-5.2, LCA

Isotype

Mouse (SJL) IgG2a, κ

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Product Citations

publications

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Compare all formats See Brilliant Violet 421™ spectral data

Technical data sheet

Product Details

Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

B10.S mouse thymocytes and splenocytes

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.

Concentration

µg sizes: 0.2 mg/ml
µl sizes: lot-specific (please contact technical support for concentration and total µg amount)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC – Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis

.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation⁴, in vivo and in vitro blocking of B cell responses^1,2, and immunohistochemical staining of acetone-fixed frozen sections³. 

Application References

(PubMed link indicates BioLegend citation)

1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
6. Pascal V, et al. 2007. J. Immunol. 179:1751.
7. Burman AC, et al. 2007. Blood 110:1064.
8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
9. Phan TG, et al. 2007. Nature Immunol. 8:992.
10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed

Product Citations

1. Sheng W et al. 2018. Cell. 174(3):549-563 . PubMed
2. Garg G et al. 2019. Cell reports. 26(7):1854-1868 . PubMed
3. Van Braeckel-Budimir N, et al. 2018. Cell Rep. 24:3374. PubMed
4. Engblom C, et al. 2017. Science. 358:6367. PubMed
5. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
6. Knox T, et al. 2019. Sci Rep. 9:6136. PubMed
7. Yue X, et al. 2019. Nat Commun. 10:2011. PubMed
8. Yang H, et al. 2019. J Neuroinflammation. 16:169. PubMed
9. Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed
10. Christopher S Garris et al. 2018. Immunity. 49(6):1148-1161 . PubMed
11. Chopin M et al. 2018. Immunity. 50(1):77-90 . PubMed
12. Juttukonda LJ, et al. 2017. Cell Host Microbe. 22:531. PubMed
13. Urata S, et al. 2018. PLoS Pathog. 14:e1007172. PubMed
14. Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed
15. Rosenbaum SR, et al. 2020. Cell Rep. 30:510. PubMed
16. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
17. Tribble JR, et al. 2020. Mol Brain. 13:81. PubMed
18. Kinder JM, et al. 2020. Cell Reports. 31(12):107784.. PubMed
19. Fukushima K, et al. 2020. Immunity. 52(3):542-556. PubMed
20. Wilkinson AC, et al. 2020. Nature Protocols. 15(2):628-648.. PubMed
21. Wilkinson AC, et al. 2019. Nature. 571:117. PubMed
22. Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed
23. Lemaître F, et al. 2013. J Immunol. 191:1578. PubMed
24. Allman W, et al. 2015. Proc Natl Acad Sci U S A. 112: 4094 – 4103. PubMed
25. Wiesner D, et al. 2016. J Immunol. 196: 365 – 374. PubMed
26. Peter Morawski, Chen-Feng Qi, Silvia Boll 2017. Sci Rep. 7:40838. PubMed
27. Iwanami N, et al. 2020. iScience. 23:101260. PubMed
28. Senatus L, et al. 2020. JCI Insight. 5:00. PubMed
29. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
30. Kawakami R, et al. 2021. Immunity. 54(5):947-961.e8. PubMed

RRID

AB_10900256 (BioLegend Cat. No. 109831)
AB_2565511 (BioLegend Cat. No. 109832)

Antigen Details

Structure

Protein tyrosine phosphatase (PTP) family, 180-240 kD

Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function

Phosphatase, T and B cell activation

Ligand/Receptor

Galectin-1, CD2, CD3, CD4

Biology Area

Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers

Molecular Family

CD Molecules

Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID

19264 View all products for this Gene ID

UniProt

View information about CD45.2 on UniProt.org

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Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Other Formats

View All CD45.2 Reagents Request Custom Conjugation