Culture system containing KBMTM GoldTM Basal Medium (00192151) and KGMTM GoldTM SingleQuotsTMsupplements (00192152) required for growth of human keratinocytes.
包含人类角质形成细胞生长所需的 KBMTM GoldTM 基础培养基 (00192151) 和 KGMTM GoldTM SingleQuotsTM 补充剂 (00192152) 的培养系统。
Product Overview
A new robust and versatile serum-free medium formulation for high and low density NHEK-Normal Human Epidermal Keratinocyte cultures
KGM-GoldTM Keratinocyte Growth Medium is designed to support both clonal growth and high density keratinocyte proliferation. The medium supports superior growth and morphology of keratinocytes isolated in KGMTM-2, KGMTM and Competitor A’s Medium (data shown in the data tab)
Furthermore, quality control at Lonza requires that keratinocytes pass strict morphological criteria specifying the minimal presence of enlarged and vacuolated cells, enabling Lonza keratinocytes to be used by the researcher for ≥15 Population Doublings.
Purchase our Adult Keratinocyte Cell Culture Kit (catalog no. 192627B) and get a discounted price on cells and media. The kit consists of a cryopreserved ampule of adult keratinocytes (catalog no. 192627) and KGMTM-Gold BulletKitTM Growth Media (catalog no. 192060)
用于高密度和低密度 NHEK-Normal 人表皮角质形成细胞培养的新型稳健且多功能的无血清培养基配方
KGM-GoldTM 角质形成细胞生长培养基旨在支持克隆生长和高密度角质形成细胞增殖。 该培养基支持在 KGMTM-2、KGMTM 和竞争对手 A 的培养基中分离的角质形成细胞的出色生长和形态（数据显示在数据选项卡中）
此外，Lonza 的质量控制要求角质形成细胞通过严格的形态学标准，规定最小存在扩大和空泡细胞，使研究人员能够将 Lonza 角质形成细胞用于≥15 人口倍增。
购买我们的成人角质形成细胞培养试剂盒（目录号 192627B）并获得细胞和培养基的折扣价。 该试剂盒由冷冻保存的成人角质形成细胞安瓿（货号 192627）和 KGMTM-Gold BulletKitTM 生长培养基（货号 192060）组成
Content & Storage
Content
1 x KBMTM GoldTM Basal Medium (00192151), 500 mL
1 x KGMTMGoldTM SingleQuotsTM Supplement pack (00192152) containing:
1 x Natural Cap Vial with Hydrocortisone, 0.50 mL
1 x Natural Cap Vial with Transferrin, 0.50 mL
1 x Amber Vial with Epinephrine, 0.25 mL
1 x Red Cap Vial with GA-1000, 0.50 mL
1 x Orange Cap Vial with BPE, 2.00 mL
1 x Green Cap Vial with hEGF, 0.50 mL
1 x Lilac Cap Vial with Insulin, 0.50 mL
Related Products
KBM™ Gold 角质形成细胞生长基础培养基 00192151
KGM™ Gold 角质形成细胞生长培养基 SingleQuots™ 补充剂和生长因子 00192152
Data
Morphology of Lonza’s Adult Keratinocytes Grown in KGM™ Gold Growth Medium Compared to Competitor A’s Adult Keratinocytes in Their Medium.
The growth performance and longevity of Competitor A’s adult keratinocytes grown in Competitor A’s Medium were compared with Lonza’s adult keratinocytes grown in KGM-Gold™ Growth Medium. Although Competitor A claims ≥25 total population doublings for their adult keratinocytes, actual results show that this lot barely obtained 19 PD. Note the presence of several enlarged cells in Passage 2 in Competitor’s Medium. At the end of passage 4 (15 PD) Lonza’s adult keratinocytes consist of mostly small proliferative cells.
