Cryopreserved ampule of Human Umbilical Vein Endothelial Cells (HUVEC) from pooled donors, containing ≥ 500,000 cells
Product Overview
Lonza’s Cryopreserved HUVECs are shipped as frozen primaries. They are guaranteed to express CD31/105 and survive in culture through 15* population doublings (*except HUVEC-XLTM guaranteed through 5 PD).
All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.
Our pooled vs. single donor options allow researchers to study or exclude donor variability from their experiments. Depending upon the study, pooled versions are typically utilized to reduce donor variability while single donor options allow researchers to assess donor-specific differences.
HUVEC-XL size consists of 10 million cells/amp achieved by pooling donors. This option is designed to support studies that require large volume of cells while still offering cost-benefits to researchers.
Lonza 的冷冻保存的 HUVEC 作为冷冻初级产品运输。 它们保证表达 CD31/105 并通过 15* 种群倍增在培养中存活(* HUVEC-XLTM 除外,保证通过 5 PD)。
所有细胞的支原体、细菌、酵母和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书。
我们的汇集与单一供体选项允许研究人员在他们的实验中研究或排除供体变异性。 根据研究,汇总版本通常用于减少捐赠者的可变性,而单一捐赠者选项允许研究人员评估特定捐赠者的差异。
HUVEC-XL 大小由 1000 万个细胞/安培组成,通过汇集供体实现。 此选项旨在支持需要大量细胞的研究,同时仍为研究人员提供成本效益。
Not All HUVECs Are Created Equal – Which Type Is Suitable for Your Research?
- CC-2517 (single donor), CC-2519 (pooled donor) – Both isolated in EGMTM Media, in absence of defined growth factors. Contains BBE. No exogenous VEGF.
- CC-2935 (single donor) – Isolated in EGMTM – Plus Media: Improved version of part number CC-2517. Offers faster proliferation rates than HUVEC isolated in EGMTM Media. No exogenous VEGF.
- C2517A (single donor), C2519A (pooled donor), 00191027 (XL size) – Isolated in EGMTM-2 Media, in presence of growth factors such as VEGF, rhFGF, rhEGF, and others. Does not contain BBE.
- C2517AS (single donor), C2519AS (pooled donor) – Pre-screened HUVECs in EGMTM-2 Media. Screened for angiogenesis markers and test positive for expression of eNOS, Axl, Tie-2 and VEGFr2.
Benefits
- Guaranteed through 15 population doublings
- Single or pooled donors available
- All cells test negative for mycoplasma, bacteria, yeast, and fungi
- HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
- Certificate of Analysis provided for every cell lot
Applications
- Would healing
- Angiogenesis
- Tissue engineering
- Inflammation
- Oncology
- Pharmacology
- Vascular modeling
- Transfection
Content & Storage
Content
1 x Cryopreserved ampule HUVEC (pooled donor) containing ≥500,000 cells
P3 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-3032
P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3010
HUVEC – 人脐静脉内皮细胞,单一供体,在 EGM™-2 中,预先筛选血管生成 C2517AS
HUVEC – 人脐静脉内皮细胞,混合,在 EGM™-2 C2519A 中
HUVEC – 人脐静脉内皮细胞,汇集在 EGM™-2 中,预先筛选血管生成 C2519AS
HUVEC – 人脐静脉内皮细胞,汇集,在 EGM™ CC-2519 中
HUVEC – EGM™-Plus CC-2935 中不含 VEGF 的人脐静脉内皮细胞,单一供体