lonza,CC-2553,RPTEC – 人肾近端小管上皮细胞

Product Overview

Lonza’s Human Renal Cells include cells isolated from various normal human kidney tissue specific to each cell type.  Human Renal Proximal Tubule Cells are isolated from the proximal tubule and are ideal for drug transporter studies. Human Renal Epithelial Cells (HRE) are comprised of cells from the cortex and glomerular region. Human Renal Cortical Cells (HRCE) are from proximal and distal tubules. Human Mesangial Cells (HMC) are isolated from the renal glomerulus and modified smooth muscle cells between capillaries.

Renal epithelial cells display a polarized morphology, which is essential for their function. They reabsorb nearly all of the glucose and amino acids in the glomerular filtrate, while allowing other substances of no nutritional value to be excreted in the urine. Physiological processes like osmoregulation and excretion can be studied with HRE in vitro.  Human renal epithelial cells are also capable of internalizing bacteria and are located near injury sites in a variety of congenital, metabolic and inflammatory diseases.

Lonza 的人肾细胞包括从对每种细胞类型特异的各种正常人肾组织中分离出来的细胞。人肾近端小管细胞从近端小管中分离出来，是药物转运蛋白研究的理想选择。人肾上皮细胞 (HRE) 由来自皮质和肾小球区域的细胞组成。人肾皮质细胞 (HRCE) 来自近端和远端小管。人系膜细胞 (HMC) 从肾小球和毛细血管之间的修饰平滑肌细胞中分离出来。

肾上皮细胞表现出极化形态，这对其功能至关重要。它们重新吸收肾小球滤液中几乎所有的葡萄糖和氨基酸，同时允许其他没有营养价值的物质从尿液中排出。渗透调节和排泄等生理过程可以在体外用 HRE 进行研究。人肾上皮细胞也能够内化细菌，并且位于各种先天性、代谢和炎症性疾病的损伤部位附近。

Select References with Lonza’s Renal Cells and Media

• Renal Physiology: Intercellular communication is a key process in the development and maintenance of multicellular organisms. Study mentioned here reported extensive spontaneous intercellular exchange of cargo vesicles and organelles between primary human proximal tubular epithelial cells. Sophie Domhan,  Lili Ma, Albert Tai, Zachary Anaya, Afshin Beheshti, Martin Zeier, Lynn Hlatky, Amir Abdollahi. Intercellular Communication by Exchange of Cytoplasmic Material via Tunneling Nano-Tube Like Structures in Primary Human Renal Epithelial Cells. PLoS ONE (2011); 6(6): e21283
• Nephrotoxicity study was done to evaluate the direct toxicity of Streptozotocin on human renal proximal tubule epithelial cells which resulted in upregulation of the activated form of the endoplasmic reticulum stress (ER stress) in the renal epithelial cells. Uetake R, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Iesato Y, Yoshizawa T, Koyama T, Yang L, Toriyama Y, Yamauchi A, Igarashi K, Tanaka M, Kuwabara T, Mori K, Yanagita M, Mukoyama M, Shindo T. Adrenomedullin-RAMP2 system suppresses ER stress-induced tubule cell death and is involved in kidney protection. PLoS ONE (2014); 9(2): e87667
• Renal Drug Transporters: Study was done using Lonza’s human renal proximal tubule cells (CC-2553), where the effect of Cobicistat (COBI) on the cytotoxicity of  Tenofovir (TFV) in renal cell cultures was examined to elucidate renal drug-drug interaction between these two components. Kirsten M. Stray, Rujuta A. Bam, Gabriel Birkus, Jia Hao, Eve-Irene Lepist, Stephen R. Yant, Adrian S. Ray, Tomas Cihlar.  Evaluation of the effect of cobicistat on the in vitro renal transport and cytotoxicity potential of tenofovir. Antimicrobial Agents and Chemotherapy (2013); 57(10): 4982-4989