lonza,lonza 00195769,KGM™ Gold 角质形成细胞生长培养基 BulletKit™

Catalog #: 00195769
KGMTM Gold Keratinocyte BulletKitTM, Calcium Free
KGMTM Gold Keratinocyte BulletKitTM,无钙

Product Overview

A new robust and versatile serum-free medium formulation for high and low density NHEK-Normal Human Epidermal Keratinocyte cultures.

KGM-GoldTM Calcium Free Keratinocyte Growth Medium is designed to support both clonal growth and high density keratinocyte proliferation. The medium supports superior growth and morphology of keratinocytes.

Furthermore, quality control at Lonza requires that keratinocytes pass strict morphological criteria specifying the minimal presence of enlarged and vacuolated cells, enabling Lonza keratinocytes to be used by the researcher for ≥15 Population Doublings.

一种新的稳健且多功能的无血清培养基配方,适用于高密度和低密度 NHEK-Normal 人类表皮角质形成细胞培养物。

KGM-GoldTM 无钙角质形成细胞生长培养基旨在支持克隆生长和高密度角质形成细胞增殖。 该培养基支持角质形成细胞的卓越生长和形态。

此外,Lonza 的质量控制要求角质形成细胞通过严格的形态学标准,规定最小存在扩大和空泡细胞,使研究人员能够将 Lonza 角质形成细胞用于≥15 人口倍增。

Content & Storage

Content

1 x Bottle Basal Medium 500 mL
1 x Natural Cap Vial with Hydrocortisone 0.50 mL
1 x Natural Cap Vial with Transferrin 0.50 mL
1 x Amber Vial with Epinephrine 0.25 mL
1 x Red Cap Vial with GA-1000 0.50 mL
1 x Orange Cap Vial with BPE 2.00 mL
1 x Green Cap Vial with hEGF 0.50 mL
1 x Lilac Cap Vial with Insulin 0.50 mL

Instructions, SDS, CoA

KGM™ Gold Keratinocyte Growth Medium BulletKit™, Calcium Free

Safety Data Sheets (SDS)

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Data

KGM™ Gold Keratinocyte Growth Medium BulletKit™, Calcium Free

Morphology of Lonza’s Adult Keratinocytes Grown in KGM™ Gold Growth Medium Compared to Competitor A’s Adult Keratinocytes in Their Medium.

The growth performance and longevity of Competitor A’s adult keratinocytes grown in Competitor A’s Medium were compared with Lonza’s adult keratinocytes grown in KGM-Gold™ Growth Medium. Although Competitor A claims ≥25 total population doublings for their adult keratinocytes, actual results show that this lot barely obtained 19 PD. Note the presence of several enlarged cells in Passage 2 in Competitor’s Medium. At the end of passage 4 (15 PD) Lonza’s adult keratinocytes consist of mostly small proliferative cells.

KGM™ Gold Keratinocyte Growth Medium BulletKit™, Calcium Free
将竞争对手 A 的成年角质形成细胞在竞争对手 A 的培养基中生长的生长性能和寿命与龙沙在 KGM-Gold™ 生长培养基中生长的成年角质形成细胞进行了比较。 尽管竞争对手 A 声称其成年角质形成细胞的总种群倍增 ≥ 25,但实际结果表明,该批次几乎没有获得 19 个 PD。 注意竞争对手培养基中第 2 段中存在几个扩大的细胞。 在第 4 代(15 PD)结束时,Lonza 的成人角质形成细胞主要由小的增殖细胞组成。

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