Cryopreserved ampule of Irradiated Mouse Embryonic Fibroblasts containing ≥2 million cells
Product Overview
Irradiated Mouse primary embryonic fibroblasts (iMEF) are dissociated from day 14 and 15 CD1 mouse embryos, expanded and then inactivated using gamma radiation.
Each vial contains at least 2 million cells. iMEF are convenient and easy to use “feeder cells” commonly co-cultured with embryonic and pluripotent stem cell cultures.
Recommended Media: Lonza’s DMEM High Glucose w/ L-Gln, 500 mL (Part 12-604F) containing 10% FBS (Sigma-Aldrich Part F6178)
经辐照的小鼠原代胚胎成纤维细胞 (iMEF) 从第 14 天和第 15 天的 CD1 小鼠胚胎中分离出来，扩增然后使用伽马辐射灭活。
每个小瓶包含至少 200 万个细胞。 iMEF 是方便且易于使用的“饲养细胞”，通常与胚胎和多能干细胞培养物共同培养。
推荐培养基：Lonza’s DMEM High Glucose w/ L-Gln，500 mL（第 12-604F 部分），含 10% FBS（Sigma-Aldrich 第 F6178 部分）
Benefits
- Ready-To-Use
- No tissue dissection or animal handling required
- Increased data reproducibility
- Characteristic morphology and staining tests confirm tissue origin and cell type
- Negative for mycoplasma, bacteria, yeast and fungi
- Certificate of Analysis (CoA) is available for every batch
Applications
- Serve as “feeder cell” monolayer in embryonic (ES) and induced pluripotent (iPSC) stem cell cultures
- Secrete important growth factors into culture medium to support ES and iPSC undifferentiated state
- Act as cellular matrix to support ES cell growth
- 在胚胎 (ES) 和诱导多能 (iPSC) 干细胞培养物中充当“饲养细胞”单层
将重要的生长因子分泌到培养基中以支持 ES 和 iPSC 未分化状态
作为细胞基质支持 ES 细胞生长
Content & Storage
Content
≥ 2 million cryopreserved, gamma irradiated cells per ampule
Safety Data Sheets (SDS)
View SDS
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
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