CellTag™ 520 Stain for In-Cell Western™ Assays,CellTag® 用于 In-Cell Western™ 的 520 染色剂； 化验

Reagents > CellTag™ 520 Stain for In-Cell Western™ Assays

CellTag™ 520 Stain for In-Cell Western™ Assays

CellTag 520 Stain is a visible fluorescent, non-specific cell stain that provides accurate normalization to cell number for In-Cell Western™ Assay applications.

The stain accumulates in both the nucleus and cytoplasm of permeabilized cells, and provides linear fluorescent signal across a wide range of cell types and cell numbers.

CellTag 520 Stain is detected in the 520 nm channel. CellTag 520 Stain is applied to the cells during incubation with an IRDye® Secondary Antibodies (e.g., IRDye 680RD or IRDye 800CW), and enables accurate measurement of target protein levels with much higher throughput than Western blotting.

CellTag 520 Stain 是一种可见的荧光、非特异性细胞染料，可为 In-Cell Western™ Assay 应用提供准确的细胞数标准化。

染色剂在透化细胞的细胞核和细胞质中积累，并在广泛的细胞类型和细胞数量中提供线性荧光信号。

在 520 nm 通道中检测到 CellTag 520 Stain。 CellTag 520 Stain 在与 IRDye® 二抗（例如 IRDye 680RD 或 IRDye 800CW）一起孵育期间应用于细胞，能够以比蛋白质印迹高得多的通量准确测量靶蛋白水平。

Properties

  • Size: 2 x 50 nmole (lyophilized)
  • λEx/λEm: 548/563 nm
  • Recommended concentration: 0.1 μM

Linear Relationship Between Fluorescence and Cell Number

CellTag™ 520 Stain for In-Cell Western™ Assays
Two-fold serial dilutions of NIH3T3 cells were plated in a 96-well plate. Cells were fixed, permeabilized, stained with CellTag 520 Stain (0.1 μM), and detected with the Odyssey M Imaging System.

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