LI-COR-IRDye® 680RD 羧酸盐,IRDye® 680RD Carboxylate

Reagents > IRDye® 680RD Carboxylate

IRDye® 680RD Carboxylate

Assays that use IRDye 680RD conjugates (such as small animal imaging and cell binding assays) may require a “dye-only” control for potential effects or retention of the dye.

Carboxylate (non-reactive) form of IRDye 680RD is an ideal control.

Note: The carboxylate dye has no reactive group and cannot be used for labeling.

使用 IRDye 680RD 偶联物的测定（例如小动物成像和细胞结合测定）可能需要“仅染料”对照来控制染料的潜在影响或保留。

IRDye 680RD 的羧酸盐（非反应性）形式是理想的对照。

注意：羧酸染料没有反应基团，不能用于标记。

How is IRDye 680RD Carboxylate Used?

In vitro Cell-based Assays

As a control for cell-based assays that monitor binding of a dye-labeled agent.

  • Validation of optical agents for in vivo administration
  • Evaluation of binding specificity

In vivo Imaging

For evaluation of behavior and clearance of the dye itself.

  • Timing of dye clearance from the animal’s body
  • Retention of dye in certain organs or sites (e.g., liver or kidneys)

Labeling Reaction Reference

As a standard to determine the amount of unreacted (“free”) dye after IRDye 680RD conjugation and purification.

Residual unreacted dye may cause:

  • Artificially high values when dye/protein (D/P) ratio is calculated
  • Increased background fluorescence in biological assays
  • 计算染料/蛋白质 (D/P) 比率时的人为高值
    生物测定中增加的背景荧光

Dye Structure

IRDye® 680RD Carboxylate

Properties

  • Chemical Formula: C39H41CIN3Na3O11S3
  • Molecular Weight: 928.37 g/mol
  • Exact Mass: 927.13

发表评论