LI-COR IRDye® 800CW BoneTag™光学探头,IRDye 800CW 近红外染料

Reagents > IRDye® 800CW BoneTag™ Optical Probe,IRDye® 800CW BoneTag™光学探头

IRDye® 800CW BoneTag™ Optical Probe

IRDye 800CW BoneTag Optical Probe is a calcium-chelating compound conjugated to IRDye 800CW near-infrared dye. Using IRDye NIR dyes extends fluorescence signal detection to the near-infrared fluorescent region of the spectrum without affecting the compound’s ability to function as a marker of the mineralization process. NIR fluorescence detection improves depth of penetration due to low tissue autofluorescence, translating to low background interference.

Calcium-chelating compounds have been used effectively for the detection of bone mineralization, growth, and morphological changes, including tetracycline derivatives, xylenol orange, alizarin, calcein, and fluorescein. NIR-labeled pamidronate has also been successfully used for detection of arterial calcification in non-invasive optical imaging1.

The ability to visualize bone anatomy and structures for an extended period facilitates use of the IRDye BoneTag in conjunction with a second optical agent specific for a primary target (i.e., tumor tissue). In addition, IRDye 800CW BoneTag may prove effective for studies dealing with bone metastasis and bone remodeling studies.

IRDye 800CW BoneTag 光学探针是一种与 IRDye 800CW 近红外染料结合的钙螯合化合物。使用 IRDye NIR 染料将荧光信号检测扩展到光谱的近红外荧光区域，而不会影响化合物作为矿化过程标记的能力。 NIR 荧光检测由于组织自发荧光低而提高了穿透深度，从而转化为低背景干扰。

钙螯合化合物已被有效地用于检测骨矿化、生长和形态变化，包括四环素衍生物、二甲酚橙、茜素、钙黄绿素和荧光素。 NIR 标记的帕米膦酸盐也已成功用于检测无创光学成像中的动脉钙化1。

长时间可视化骨骼解剖和结构的能力有助于将 IRDye BoneTag 与特定于主要目标（即肿瘤组织）的第二种光学试剂结合使用。此外，IRDye 800CW BoneTag 可能证明对处理骨转移和骨重塑研究的研究有效。

Learn about other LI-COR optical probes.

Applications

  • Microscopy
  • Bone Imaging

Absorbance and Emission Spectra

IRDye® 800CW BoneTag™ Optical Probe

Data Example

IRDye® 800CW BoneTag™ Optical Probe
Bone-targeted binding was examined using MC3T3 (osteoblast) cells in a cell-based assay. MC3T3 cells exhibited a dose dependent increase in signal when incubated with IRDye 800CW BoneTag. Incubation of labeled BoneTag with A431 cells (epidermoid carcinoma), a non-osteoblast cell line, showed low non-specific binding.
在基于细胞的测定中使用 MC3T3（成骨细胞）细胞检查骨靶向结合。 当与 IRDye 800CW BoneTag 一起孵育时，MC3T3 细胞表现出剂量依赖性的信号增加。 标记的 BoneTag 与非成骨细胞系 A431 细胞（表皮样癌）的孵育显示出低非特异性结合。

References

  1. Zaheer A et al. (2001) In vivo near-infrared fluorescence imaging of osteoblastic activity. Nat Biotechnol, 19. 1148-54.
  2. Kovar, J. L., Xu, X., Draney, D., Cupp, A., Simpson, M. A., & Michael Olive, D. (2011). Anal Biochem, 416(2), 167–173. doi: 10.1016/j.ab.2011.05.011.

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