Reagents > IRDye® 800CW Donkey anti-Rabbit IgG Secondary Antibody,IRDye® 800CW 驴抗兔 IgG 二抗
Immunogen
Rabbit IgG
Purity and Specificity
The antibody was isolated by affinity chromatography using antigens coupled to agarose beads. Based on ELISA, this antibody reacts with the heavy and light chains of rabbit IgG, and with the light chains common to most rabbit immunoglobulins. This antibody was tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, and sheep serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications.
使用与琼脂糖珠偶联的抗原通过亲和层析分离抗体。 基于 ELISA，该抗体与兔 IgG 的重链和轻链以及大多数兔免疫球蛋白常见的轻链发生反应。 该抗体通过 ELISA 和/或固相吸附测试，以确保与牛、鸡、山羊、豚鼠、仓鼠、马、人、小鼠、大鼠和绵羊血清蛋白的交叉反应最小，但可能与 来自其他物种的免疫球蛋白。 该偶联物经过专门测试，可用于蛋白质印迹和 In-Cell Western™ 检测应用。
Applications
Highly recommended for:
- Western Blot
- In-Cell Western Assay
- On-Cell Western Assay
- FRET-Based Assays
- Protein Array
- Immunohistochemistry
- Small Animal Imaging
- Microscopy
- 2D Gel Detection
- Tissue Section Imaging
- Virus Titration Assay
- 蛋白质印迹
细胞内西方检测
On-Cell Western 检测
基于 FRET 的检测
蛋白质阵列
免疫组织化学
小动物成像
显微镜
2D 凝胶检测
组织切片成像
病毒滴定法
Formulation
IRDye 800CW secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.
Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.
IRDye 800CW 二抗以纯化的免疫球蛋白偶联物形式提供，在 pH 7.4 的磷酸盐缓冲盐水中冻干。 避光。 在重组前储存在 4°C。
复溶后，每瓶含有 10 mg/mL BSA（不含 IgG 和蛋白酶）作为稳定剂和 0.01% 叠氮化钠作为防腐剂。 按照指示重新配制时，浓度为 1.0 mg/mL。 有关重组的详细信息，请参阅包装插页。
Recommended Dilutions
| Application | Recommended | Suggested Range |
|---|---|---|
| Odyssey® Western blot detection | 1:15,000 | 1:5,000 – 1:25,000 |
| In-Cell Western assay detection | 1:800 | 1:200 – 1:800 |
| Other | User optimized |
Optimum dilutions will vary and should be determined empirically.
RRID
- P/N 925-32213: RRID AB_2715510
- P/N 926-32213: RRID AB_621848