LI-COR IRDye® 800CW 山羊抗小鼠 IgG 二抗,LI-COR IRDye 800CW 二抗

Reagents > IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody,IRDye® 800CW 山羊抗小鼠 IgG 二抗

IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody

Immunogen

Mouse IgG paraproteins

Purity and Specificity

Isolation of specific antibodies was accomplished by affinity chromatography using pooled mouse IgG covalently linked to agarose. Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, and IgG3, and with the light chains of mouse IgM and IgA. This antibody was tested by dot blot and and/or solid-phase adsorbed for minimal cross-reactivity with human, rabbit, goat, rat, and horse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.

使用与琼脂糖共价连接的混合小鼠 IgG 通过亲和层析实现特异性抗体的分离。 基于 ELISA 和流式细胞术，该抗体与小鼠 IgG1、IgG2a、IgG2b 和 IgG3 的重链和轻链以及小鼠 IgM 和 IgA 的轻链反应。 通过斑点印迹和/或固相吸附测试该抗体与人、兔、山羊、大鼠和马血清蛋白的交叉反应最小，但可能与来自其他物种的免疫球蛋白交叉反应。 该偶联物经过专门测试，可用于蛋白质印迹应用。

Applications

Highly recommended for:

  • Western Blot
  • In-Cell Western™ Assay
  • On-Cell Western Assay
  • Protein Array
  • Immunohistochemistry
  • Small Animal Imaging
  • Microscopy
  • 2D Gel Detection
  • Tissue Section Imaging
  • Virus Titration Assay
  • 蛋白质印迹
    In-Cell Western™ 检测
    On-Cell Western 检测
    蛋白质阵列
    免疫组织化学
    小动物成像
    显微镜
    2D 凝胶检测
    组织切片成像
    病毒滴定法

Formulation

IRDye 800CW secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

IRDye 800CW 二抗以纯化的免疫球蛋白偶联物形式提供，在 pH 7.4 的磷酸盐缓冲盐水中冻干。 避光。 在重组前储存在 4°C。

复溶后，每瓶含有 10 mg/mL BSA（不含 IgG 和蛋白酶）作为稳定剂和 0.01% 叠氮化钠作为防腐剂。 按照指示重新配制时，浓度为 1.0 mg/mL。 有关重组的详细信息，请参阅包装插页。

Recommended Dilutions

Application Recommended Suggested Range
Odyssey Western blot detection 1:15,000 1:5,000 – 1:25,000
In-Cell Western assay detection 1:200 1:200 – 1:1,200
Other User optimized

Optimum dilutions will vary and should be determined empirically.

RRID

  • P/N 925-32210: RRID AB_2687825
  • P/N 926-32210: RRID AB_621842

Example Data

IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody
Western blot of cellular fractions from Staurosporine (STS) treated and non-treated Hela cells. Lane 1: Odyssey® One-Color Molecular Weight Marker (P/N 928-40000); Lane 2: human heart mitochondrial control (Mitosciences MS801-50); Lanes 4-6: control fractions (cytosolic, mitochondrial, and nuclear); Lanes 8-10: apoptotic (STS-treated) fractions (cytosolic, mitochondrial, and nuclear). Lysates were resolved on a 4-12% Bis-Tris gel and transferred to Odyssey Nitrocellulose (P/N 926-31090). Membrane was blocked with Odyssey Blocking Buffer (P/N 927-40000) and probed with ApoTrack mAb cocktail followed by detection with IRDye 800CW Goat anti-Mouse IgG (P/N 926-32210). Note: Cytochrome c is not present in the control cytosolic fraction and is present in the apoptotic cytosolic fraction.
来自星形孢菌素 (STS) 处理和未处理的 Hela 细胞的细胞组分的蛋白质印迹。 泳道 1：Odyssey® 单色分子量标记（P/N 928-40000）； 泳道 2：人心脏线粒体控制（Mitosciences MS801-50）； 泳道 4-6：控制部分（细胞溶质、线粒体和细胞核）； 泳道 8-10：凋亡（STS 处理）部分（细胞溶质、线粒体和细胞核）。 裂解物在 4-12% Bis-Tris 凝胶上分离并转移到 Odyssey Nitrocellulose (P/N 926-31090)。 用 Odyssey 封闭缓冲液 (P/N 927-40000) 封闭膜，用 ApoTrack mAb 混合物探测，然后用 IRDye 800CW 山羊抗小鼠 IgG (P/N 926-32210) 检测。 注意：细胞色素 c 不存在于对照细胞溶质部分中，而是存在于凋亡细胞质部分中。

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