Reagents > IRDye® 800CW Goat anti-Mouse IgM (µ chain specific) Secondary Antibody,IRDye® 800CW 山羊抗小鼠 IgM(μ 链特异性)二抗
Immunogen
Mouse IgM paraproteins
Purity and Specificity
Isolation of specific antibodies was accomplished by affinity chromatography using mouse IgM covalently linked to agarose. Based on ELISA and/or flow cytometry, this antibody reacts with the heavy chain of mouse IgM. This antibody has been tested by dot blot and/or solid-phase adsorbed to ensure minimal cross-reactivity with mouse IgG1, IgG2a, IgG3, and IgA, pooled human sera and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot applications.
使用与琼脂糖共价连接的小鼠 IgM 通过亲和层析实现特异性抗体的分离。 基于 ELISA 和/或流式细胞术,该抗体与小鼠 IgM 的重链发生反应。 该抗体已通过斑点印迹和/或固相吸附测试,以确保与小鼠 IgG1、IgG2a、IgG3 和 IgA、合并的人血清和纯化的人副蛋白的交叉反应最小。 该偶联物经过专门测试,可用于蛋白质印迹应用。
Applications
Highly recommended for:
- Western Blot
- In-Cell Western™ Assay
- On-Cell Western Assay
- Protein Array
- Immunohistochemistry
- Small Animal Imaging
- Microscopy
- 2D Gel Detection
- Tissue Section Imaging
- Virus Titration Assay
- 蛋白质印迹
In-Cell Western™ 检测
On-Cell Western 检测
蛋白质阵列
免疫组织化学
小动物成像
显微镜
2D 凝胶检测
组织切片成像
病毒滴定法
Formulation
IRDye 800CW secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.
Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.
IRDye 800CW 二抗以纯化的免疫球蛋白偶联物形式提供,在 pH 7.4 的磷酸盐缓冲盐水中冻干。 避光。 在重组前储存在 4°C。
复溶后,每瓶含有 10 mg/mL BSA(不含 IgG 和蛋白酶)作为稳定剂和 0.01% 叠氮化钠作为防腐剂。 按照指示重新配制时,浓度为 1.0 mg/mL。 有关重组的详细信息,请参阅包装插页。
Recommended Dilutions
Application | Recommended | Suggested Range |
---|---|---|
Odyssey® Western blot detection | 1:15,000 | 1:5,000 – 1:25,000 |
Other | User optimized |
Optimum dilutions will vary and should be determined empirically.
RRID
- P/N 925-32280: RRID AB_2814916
- P/N 926-32280: RRID AB_2814919