LI-COR IRDye® 800CW 山羊抗大鼠 IgG 二抗, LI-COR IRDye® 800CW 二抗

Immunogen

Rat IgG, whole molecule

Purity and Specificity

The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Based on immunoelectrophoresis and/or ELISA, this antibody reacts with whole molecule rat IgG, and with the light chains of other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and and/or solid-phase adsorbed to ensure minimal cross-reactivity with mouse, bovine, horse, human, and rabbit serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.

使用与琼脂糖珠偶联的抗原，通过免疫亲和层析从抗血清中纯化抗体。 基于免疫电泳和/或 ELISA，该抗体与全分子大鼠 IgG 以及其他大鼠免疫球蛋白的轻链发生反应。 未检测到针对非免疫球蛋白血清蛋白的抗体。 该抗体已通过 ELISA 和/或固相吸附测试，以确保与小鼠、牛、马、人类和兔血清蛋白的交叉反应最小，但可能与其他物种的免疫球蛋白交叉反应。 该偶联物经过专门测试，可用于蛋白质印迹应用。

Applications

Highly recommended for:

• Western Blot
• In-Cell Western™ Assay
• On-Cell Western Assay
• Protein Array
• Immunohistochemistry
• Small Animal Imaging
• Microscopy
• 2D Gel Detection
• Tissue Section Imaging
• Virus Titration Assay

Formulation

IRDye 800CW secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

IRDye 800CW 二抗以纯化的免疫球蛋白偶联物形式提供，在 pH 7.4 的磷酸盐缓冲盐水中冻干。 避光。 在重组前储存在 4°C。

复溶后，每瓶含有 10 mg/mL BSA（不含 IgG 和蛋白酶）作为稳定剂和 0.01% 叠氮化钠作为防腐剂。 按照指示重新配制时，浓度为 1.0 mg/mL。 有关重组的详细信息，请参阅包装插页。

Recommended Dilutions

Optimum dilutions will vary and should be determined empirically.

RRID

• P/N 925-32219: RRID AB_2721932
• P/N 926-32219: RRID AB_1850025

Example Data