Reagents > IRDye® 800CW Streptavidin,IRDye® 800CW 链霉亲和素
IRDye 800CW Streptavidin is supplied as a liquid in buffer containing 10 mM phosphate, 183 mM NaCl, 2.7 nM KCl, pH 7.4 with sodium azide 0.005% (w/v) as a preservative.
To use, centrifuge briefly before use to eliminate aggregates that may have formed in solution. This will reduce non-specific background staining. A final concentration of 0.2 to 1.0 µg/ml (1:1,000 to 1:5,000) is usually satisfactory for most applications; however, appropriate dilution may need to be determined empirically.
For membrane-based applications and In-Gel Westerns, it is recommended to add SDS (0.02% to 0.1% final concentration), in addition to Tween® 20 (0.1 to 0.2% final concentration) during the detection incubation step to reduce non-specific background staining.
IRDye 800CW 链霉亲和素以液体形式提供，缓冲液中含有 10 mM 磷酸盐、183 mM NaCl、2.7 nM KCl，pH 7.4，叠氮化钠 0.005% (w/v) 作为防腐剂。
要使用，请在使用前短暂离心以消除可能在溶液中形成的聚集体。 这将减少非特异性背景染色。 对于大多数应用，0.2 到 1.0 µg/ml（1:1,000 到 1:5,000）的最终浓度通常是令人满意的； 但是，可能需要根据经验确定适当的稀释度。
对于基于膜的应用和 In-Gel Westerns，建议在检测孵育步骤中添加 SDS（0.02% 至 0.1% 最终浓度）和 Tween® 20（0.1 至 0.2% 最终浓度）以减少非 特定背景染色。
Recommended Dilutions
| Application | Suggested Range | Tween 20* | SDS* |
|---|---|---|---|
| Odyssey® Western blot detection | 1:1,000 – 1:5,000 | 0.1 – 0.2% (v/v) | 0.02 – 0.1% (v/v) |
| Other | User optimized | User optimized | User optimized |
Optimum dilutions will vary and should be determined empirically.
* Added to reduce non-specific background staining.
See the complete line of IRDye Streptavidins.