Reagents > IRDye® 680LT Donkey anti-Chicken Secondary Antibody,IRDye® 680LT 驴抗鸡二抗
Immunogen
Chicken IgY, whole molecule. IgY is the original designation for the IgG-like protein found in both serum and egg yolk.
Purity and Specificity
The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule chicken IgY, and with the light chains of other chicken immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody was tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, goat, guinea pig, Syrian hamster, horse, human, mouse, rabbit, rat, and sheep serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.
使用与琼脂糖珠偶联的抗原，通过免疫亲和层析从抗血清中分离抗体。 基于免疫电泳和/或 ELISA，抗体与全分子鸡 IgY 以及其他鸡免疫球蛋白的轻链反应。 未检测到针对非免疫球蛋白血清蛋白的反应性。 该抗体通过 ELISA 和/或固相吸附测试，以确保与牛、山羊、豚鼠、叙利亚仓鼠、马、人、小鼠、兔、大鼠和绵羊血清蛋白的交叉反应最小，但可能发生交叉反应 与来自其他物种的免疫球蛋白。 该偶联物经过专门测试，可用于蛋白质印迹应用。
Applications
Recommended for:
- Western Blot
- Protein Array
- Immunohistochemistry
- Microscopy
- 2D Gel Detection
- Tissue Section Imaging
- 蛋白质印迹
蛋白质阵列
免疫组织化学
显微镜
2D 凝胶检测
组织切片成像
Not Recommended for:
- Small Animal Imaging
- In-Cell Western Assay
- On-Cell Western Assay
Formulation
IRDye 680LT secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.
Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.
IRDye 680LT 二抗以纯化的免疫球蛋白偶联物形式提供，在磷酸盐缓冲盐水中冻干，pH 7.4。 避光。 在重组前储存在 4°C。
复溶后，每瓶含有 10 mg/mL BSA（不含 IgG 和蛋白酶）作为稳定剂和 0.01% 叠氮化钠作为防腐剂。 按照指示重新配制时，浓度为 1.0 mg/mL。 有关重组的详细信息，请参阅包装插页。
Recommended Dilutions
| Application | Recommended | Suggested Range |
|---|---|---|
| Odyssey Western blot detection | 1:20,000 | 1:20,000 – 1:50,000 |
| Other | User optimized |
Optimum dilutions will vary and should be determined empirically.
RRID
- P/N 925-68028: RRID AB_2814923
- P/N 926-68028: RRID AB_10707008
Example Data
