LI-COR IRDye® 680LT 山羊抗小鼠 IgG 二抗,LI-COR IRDye 680LT 二抗

Reagents > IRDye® 680LT Goat anti-Mouse IgG Secondary Antibody,IRDye® 680LT 山羊抗小鼠 IgG 二抗

IRDye® 680LT Goat anti-Mouse IgG Secondary Antibody

Immunogen

Mouse IgG paraproteins

Purity and Specificity

Isolation of specific antibodies was accomplished by affinity chromatography using pooled mouse IgG covalently linked to agarose. Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, and IgG3, and with the light chains of mouse IgM and IgA. This antibody was tested by dot blot and and/or solid-phase adsorbed for minimal cross-reactivity with human, rabbit, goat, rat, and horse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.

使用与琼脂糖共价连接的混合小鼠 IgG 通过亲和层析实现特异性抗体的分离。 基于 ELISA 和流式细胞术，该抗体与小鼠 IgG1、IgG2a、IgG2b 和 IgG3 的重链和轻链以及小鼠 IgM 和 IgA 的轻链反应。 通过斑点印迹和/或固相吸附测试该抗体与人、兔、山羊、大鼠和马血清蛋白的交叉反应最小，但可能与来自其他物种的免疫球蛋白交叉反应。 该偶联物经过专门测试，可用于蛋白质印迹应用。

Applications

Recommended for:

  • Western Blot
  • Protein Array
  • Immunohistochemistry
  • Microscopy
  • 2D Gel Detection
  • Tissue Section Imaging
  • 蛋白质印迹
    蛋白质阵列
    免疫组织化学
    显微镜
    2D 凝胶检测
    组织切片成像

Not Recommended for:

  • Small Animal Imaging
  • In-Cell Western Assay
  • On-Cell Western Assay

Formulation

IRDye 680LT secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

IRDye 680LT 二抗以纯化的免疫球蛋白偶联物形式提供，在磷酸盐缓冲盐水中冻干，pH 7.4。 避光。 在重组前储存在 4°C。

复溶后，每瓶含有 10 mg/mL BSA（不含 IgG 和蛋白酶）作为稳定剂和 0.01% 叠氮化钠作为防腐剂。 按照指示重新配制时，浓度为 1.0 mg/mL。 有关重组的详细信息，请参阅包装插页。

Recommended Dilutions

Application Recommended Suggested Range
Odyssey Western blot detection 1:20,000 1:20,000 – 1:50,000
Other User optimized

Optimum dilutions will vary and should be determined empirically.

Note: When using PVDF membranes for Western blotting applications, SDS (final concentration of 0.01 – 0.02%) and Tween® 20 (final concentration of 0.1 – 0.2%) must be added during the detection incubation step to avoid non-specific background staining.

RRID

  • P/N 925-68020: RRID AB_2687826
  • P/N 926-68020: RRID AB_10706161

Example Data

IRDye® 680LT Goat anti-Mouse IgG Secondary Antibody
Western blot detection of ERK2 in Hela lysate (5 µg – 78 ng). Samples loaded in 4X protein loading buffer (P/N 928-40004), resolved on a 10% Bis-Tris gel, and transferred to Odyssey® nitrocellulose (P/N 926-31090). The membrane was probed with mouse anti-ERK2 (Santa Cruz P/N sc-1647) followed by detection with IRDye 680LT Goat anti-Mouse IgG (P/N 926-68020).
对 Hela 裂解液 (5 µg – 78 ng) 中的 ERK2 进行蛋白质印迹检测。 样品上样于 4X 蛋白上样缓冲液 (P/N 928-40004)，在 10% Bis-Tris 凝胶上分离，然后转移至 Odyssey® 硝酸纤维素 (P/N 926-31090)。 用小鼠抗 ERK2 (Santa Cruz P/N sc-1647) 探测膜，然后用 IRDye 680LT 山羊抗小鼠 IgG (P/N 926-68020) 检测。

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