LI-COR IRDye 680LT 二抗,IRDye® 680LT 山羊抗小鼠 IgM（μ 链特异性）二抗

Reagents > IRDye® 680LT Goat anti-Mouse IgM (µ chain specific) Secondary Antibody,IRDye® 680LT 山羊抗小鼠 IgM（μ 链特异性）二抗

IRDye® 680LT Goat anti-Mouse IgM (µ chain specific) Secondary Antibody

Immunogen

Mouse IgM paraproteins

Purity and Specificity

Isolation of specific antibodies was accomplished by affinity chromatography using mouse IgM covalently linked to agarose. Based on ELISA and/or flow cytometry, this antibody reacts with the heavy chain of mouse IgM. This antibody has been tested by dot blot and/or solid-phase adsorbed to ensure minimal cross-reactivity with mouse IgG1, IgG2a, IgG3, and IgA, pooled human sera and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot applications.

使用与琼脂糖共价连接的小鼠 IgM 通过亲和层析实现特异性抗体的分离。 基于 ELISA 和/或流式细胞术，该抗体与小鼠 IgM 的重链发生反应。 该抗体已通过斑点印迹和/或固相吸附测试，以确保与小鼠 IgG1、IgG2a、IgG3 和 IgA、合并的人血清和纯化的人副蛋白的交叉反应最小。 该偶联物经过专门测试，可用于蛋白质印迹应用。

Applications

Recommended for:

  • Western Blot
  • Protein Array
  • Immunohistochemistry
  • Microscopy
  • 2D Gel Detection
  • Tissue Section Imaging
  • 蛋白质印迹
    蛋白质阵列
    免疫组织化学
    显微镜
    2D 凝胶检测
    组织切片成像

Not Recommended for:

  • Small Animal Imaging
  • In-Cell Western Assay
  • On-Cell Western Assay

Formulation

IRDye 680LT secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

IRDye 680LT 二抗以纯化的免疫球蛋白偶联物形式提供，在磷酸盐缓冲盐水中冻干，pH 7.4。 避光。 在重组前储存在 4°C。

复溶后，每瓶含有 10 mg/mL BSA（不含 IgG 和蛋白酶）作为稳定剂和 0.01% 叠氮化钠作为防腐剂。 按照指示重新配制时，浓度为 1.0 mg/mL。 有关重组的详细信息，请参阅包装插页。

Recommended Dilutions

Application Recommended Suggested Range
Odyssey Western blot detection 1:20,000 1:20,000 – 1:50,000
Other User optimized

Optimum dilutions will vary and should be determined empirically.

Note: When using PVDF membranes for Western blotting applications, SDS (final concentration of 0.01 – 0.02%) and Tween® 20 (final concentration of 0.1 – 0.2%) must be added during the detection incubation step to avoid non-specific background staining.
注意：当使用 PVDF 膜进行蛋白质印迹应用时，必须在检测孵育步骤中添加 SDS（终浓度为 0.01 – 0.02%）和 Tween® 20（终浓度为 0.1 – 0.2%）以避免非特异性背景染色。

RRID

  • P/N 925-68080: RRID AB_2814917
  • P/N 926-68080: RRID AB_2814920

Example Data

IRDye® 680LT Goat anti-Mouse IgM (µ chain specific) Secondary Antibody
Western blot detection of guinea pig IgG with IRDye 680LT Donkey anti-Guinea Pig. Serial dilutions of guinea pig IgG were spiked into C32 lysate (Santa Cruz P/N sc-2205). Samples were resolved on a 10% Bis-Tris gel and transferred to Odyssey® Nitrocellulose membrane (P/N 926-31092). The membrane was blocked with Odyssey Blocking Buffer (P/N 927-40000) followed by detection with IRDye 680LT Donkey anti-Guinea Pig (P/N 926-68030).
用 IRDye 680LT Donkey anti-Guinea Pig 对豚鼠 IgG 进行蛋白质印迹检测。 将连续稀释的豚鼠 IgG 加入 C32 裂解液 (Santa Cruz P/N sc-2205)。 样品在 10% Bis-Tris 凝胶上分离并转移到 Odyssey® 硝酸纤维素膜（P/N 926-31092）上。 用 Odyssey 封闭缓冲液 (P/N 927-40000) 封闭膜，然后用 IRDye 680LT 驴抗豚鼠 (P/N 926-68030) 检测。

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