LI-COR IRDye® 680RD BoneTag™ 光学探头,LI-COR IRDye 680RD 近红外染料

Reagents > IRDye® 680RD BoneTag™ Optical Probe

IRDye® 680RD BoneTag™ Optical Probe

IRDye 680RD BoneTag Optical Probe is a calcium-chelating compound conjugated to IRDye 680RD near-infrared dye. Using IRDye NIR dyes extends fluorescence signal detection to the near-infrared fluorescent region of the spectrum without affecting the compound’s ability to function as a marker of the mineralization process. NIR fluorescence detection improves depth of penetration due to low tissue autofluorescence, translating to low background interference.

Calcium-chelating compounds have been used effectively for the detection of bone mineralization, growth, and morphological changes, including tetracycline derivatives, xylenol orange, alizarin, calcein, and fluorescein. NIR-labeled pamidronate has also been successfully used for detection of arterial calcification in non-invasive optical imaging1.

IRDye 680RD BoneTag 光学探针是一种与 IRDye 680RD 近红外染料结合的钙螯合化合物。 使用 IRDye NIR 染料将荧光信号检测扩展到光谱的近红外荧光区域，而不影响化合物作为矿化过程标记的能力。 NIR 荧光检测由于组织自发荧光低而提高了穿透深度，从而转化为低背景干扰。

钙螯合化合物已被有效地用于检测骨矿化、生长和形态变化，包括四环素衍生物、二甲酚橙、茜素、钙黄绿素和荧光素。 NIR 标记的帕米膦酸盐也已成功用于检测无创光学成像中的动脉钙化1。

Learn about other LI-COR optical probes.

Applications

  • Microscopy
  • Bone Imaging

Absorbance and Emission Spectra

IRDye® 680RD BoneTag™ Optical Probe

Data Example

IRDye® 680RD BoneTag™ Optical Probe
Image of IRDye tumor specific agent and IRDye BoneTag optical probe. The pseudocolor represents the 800 nm channel with red representing the highest level of signal. The white arrow points to the tumor while the orange arrow points to stomach signal. The grey scale image represents the IRDye BoneTag incorporated in skeletal features (700 nm image).

References

  1. Zaheer A et al. (2001) In vivo near-infrared fluorescence imaging of osteoblastic activity. Nat Biotechnol, 19. 1148-54.
  2. Kovar, J. L., Xu, X., Draney, D., Cupp, A., Simpson, M. A., & Michael Olive, D. (2011). Anal Biochem, 416(2), 167–173. doi: 10.1016/j.ab.2011.05.011.

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