Reagents > IRDye® 800CW Rabbit anti-HRP,IRDye® 800CW 兔抗 HRP
Immunogen
Peroxidase from horseradish roots
Purity and Specificity
The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with peroxidase from horseradish roots. It may cross-react with peroxidase from other sources. The conjugate has been specifically tested and qualified for Western blot applications
使用与琼脂糖珠偶联的抗原，通过免疫亲和层析从抗血清中纯化抗体。 基于免疫电泳和/或 ELISA，抗体与来自辣根的过氧化物酶反应。 它可能与其他来源的过氧化物酶发生交叉反应。 该偶联物经过专门测试，可用于蛋白质印迹应用
Application
- Western Blot
Formulation
IRDye 800CW secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Store at 4 °C prior to reconstitution.
Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.
IRDye 800CW 二抗以纯化的免疫球蛋白偶联物形式提供，在 pH 7.4 的磷酸盐缓冲盐水中冻干。 在重组前储存在 4°C。
复溶后，每瓶含有 10 mg/mL BSA（不含 IgG 和蛋白酶）作为稳定剂和 0.01% 叠氮化钠作为防腐剂。 按照指示重新配制时，浓度为 1.0 mg/mL。 有关重组的详细信息，请参阅包装插页。
Recommended Dilutions
| Application | Recommended | Suggested Range |
|---|---|---|
| Odyssey® Western blot detection | 1:15,000 | 1:5,000 – 1:25,000 |
| Other | User optimized |
Optimum dilutions will vary and should be determined empirically.