LI-COR Sapphire700™ 染色剂用于细胞活力检测,In-Cell Western™ 检测标准化

Reagents > Sapphire700™ Stain for Cell Viability Assays and In-Cell Western™ Assay Normalization,Sapphire700™ 染色剂用于细胞活力检测和 In-Cell Western™ 检测标准化

Sapphire700™ Stain for Cell Viability Assays and In-Cell Western™ Assay Normalization

Sapphire700 Stain is a non-specific cell stain that accumulates in both the nucleus and cytoplasm of fixed or dead cells, but not live cells. When used to stain serial dilutions of A431 cells in 96-well plates, Sapphire700 Stain displays linearity of fluorescent signal for higher cell densities, from ~50,000 to ~250,000 cells/well.

Simultaneous staining of cells with Sapphire700 and DRAQ5® (Biostatus, Ltd.) Stains expands the linear range, allowing more accurate normalization of cell number across both low and high cell densities.

Sapphire700 Stain 是一种非特异性细胞染料，可在固定或死细胞的细胞核和细胞质中积累，但不会在活细胞中积累。 当用于在 96 孔板中对 A431 细胞的连续稀释液进行染色时，Sapphire700 Stain 显示出荧光信号的线性，用于更高的细胞密度，从 ~50,000 到 ~250,000 个细胞/孔。

使用 Sapphire700 和 DRAQ5® (Biostatus, Ltd.) 染色剂同时染色细胞扩大了线性范围，允许在低细胞密度和高细胞密度下更准确地标准化细胞数。

Use Sapphire700 Stain and DRAQ5 Stain as Normalizing Agents for ICW Assays

Sapphire700™ Stain for Cell Viability Assays and In-Cell Western™ Assay Normalization
Sapphire700™ Stain for Cell Viability Assays and In-Cell Western™ Assay Normalization
Dilutions of A431 cells were plated on clear, flat-bottomed 96-well plates, then fixed and permeabilized. Cells were stained with DRAQ5 stain alone, Sapphire700 stain alone, or both stains combined. Top: Two-fold dilutions of cells, over a wide range of cell densities (0 – 200,000 cells/well). Bottom: Closer examination of linearity of signal over the range of 25,000 – 250,000 cells/well, in dilution increments of 25,000 cells.
将 A431 细胞的稀释液接种在透明的平底 96 孔板上，然后固定并透化。 细胞用单独的 DRAQ5 染料、单独的 Sapphire700 染料或两种染料组合染色。 上图：细胞的两倍稀释度，细胞密度范围很广（0 – 200,000 个细胞/孔）。 底部：在 25,000 – 250,000 个细胞/孔的范围内，以 25,000 个细胞的稀释增量更仔细地检查信号的线性。

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