Reagents > VRDye™ 549 Donkey anti-Rabbit IgG Secondary Antibody,VRDye™ 549 驴抗兔 IgG 二抗
Immunogen
Rabbit IgG
Purity and Specificity
The antibody was isolated by affinity chromatography using antigens coupled to agarose beads. Based on ELISA, this antibody reacts with heavy and light chains of rabbit IgG and light chains common to most rabbit immunoglobulins. This antibody has been tested by ELISA and/or solid phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, and sheep serum proteins but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.
使用与琼脂糖珠偶联的抗原通过亲和层析分离抗体。 基于 ELISA，该抗体与兔 IgG 的重链和轻链以及大多数兔免疫球蛋白常见的轻链发生反应。 该抗体已通过 ELISA 和/或固相吸附测试，以确保与牛、鸡、山羊、豚鼠、仓鼠、马、人、小鼠、大鼠和绵羊血清蛋白的交叉反应最小，但可能与免疫球蛋白发生交叉反应 来自其他物种。 该偶联物经过专门测试，可用于蛋白质印迹应用。
Applications
- Western blotting
Formulation
VRDye 549 secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C before and after reconstitution.
Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.
VRDye 549 二抗以纯化的免疫球蛋白偶联物形式提供，在 pH 7.4 的磷酸盐缓冲盐水中冻干。 避光。 在重组之前和之后储存在 4°C。
复溶后，每瓶含有 10 mg/mL BSA（不含 IgG 和蛋白酶）作为稳定剂和 0.01% 叠氮化钠作为防腐剂。 按照指示重新配制时，浓度为 1.0 mg/mL。 有关重组的详细信息，请参阅包装插页。
Recommended Dilutions
| Application | Recommended | Suggested Range |
|---|---|---|
| Western Blot Detection | 1:10,000 | 1:5,000 – 1:15,000 |
| Other | User-optimized |
Suggested working dilutions are given as a guide only. Optimum dilutions will vary and should be determined empirically.