Omalizumab
分子量：149KD
简介：Omalizumab is a recombinant, humanized, monoclonal antibody against human immunoglobulin E (IgE).
产品形式：蛋白原液 。每种装于冻存管中，单抗质量为标示数量1MG或者5MG，体积根据其浓度不同略有不同。
质量标准：
Non-reduced CE-SDS:………………………………………….>90%;
SEC-HPLC:………………………………………………………….>95%;
Bacterial Endotoxins Test：…………………………………. .<10EU/ml
Residual Proteins of Host Cell: ……………………………. <100 ng/mg
Exogenous residual DNA: …………………………………. . <100pg/mg
Residual Protein A: ……………………………………………. .<100 ng/mg
Biological Activity:………………………………………….……. .80% to 125% of standard
储存条件：-70 ± 15℃for long-term storage, 2-8℃for short-term storage, away from light.
运输条件：干冰运输
使用建议：
提供的产品为蛋白原液形式。每种装于冻存管中，单抗质量为标示数量1MG或者5MG，体积根据其浓度不同略有不同。
使用前可以根据自己实验需求进行稀释，溶剂建议用去离子无菌水(货号MA0028，去离子无菌水(细胞培养用水))或者PBS（货号MA0015 PBS(1X),细胞培养级）。
不建议使用含有防腐成分的稀释液进行稀释，会增加细胞或者动物毒性。
稀释前，计算加入多少体积溶剂，请参考购买批次质检单上蛋白浓度，或者询问我司，
举例，质检单显示该批次单抗蛋白原液蛋白浓度为 50mg/ml
对应5MG包装的蛋白原液体积为5mg（质量）/50mg/ml(浓度)=0.1ML，
实验需求蛋白浓度为10mg/ml, 需要稀释倍数为 50/10=5, 则需要加入0.4ML,即400ul PBS或者无菌去离子水即可。
若给药量为固定质量值，比如单次给药0.5MG，对应蛋白原液给药体积为10ul，建议稀释后，提高给药体积降低误差，因为注射器或者移液器吸头残留会影响实验效果。
稀释后的蛋白溶液，可于2-8度冷藏避光保存不超过2周，不建议长期储存。
避免反复冻融，每次冻融蛋白活性会降低约5%左右。
生物活性（仅来自于公开文献，不保证其有效性）
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描述
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Omalizumab is a recombinant, humanized, monoclonal antibody against human immunoglobulin E (IgE). |
|
靶点 |
IgE |
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体外 |
Tonsillar B cells at a concentration of 1 × 106/mL in supplemented RPMI1640 were treated with 400 U/mL recombinant IL-4 plus 1 μg/mL anti-CD40 in the presence or absence of omalizumab or control antibody (human IgGκ). Omalizumab was reconstituted in sterile PBS at a concentration of 15 mg/mL. Dilutions of omalizumab were made in RPMI1640. In some instances the cells were pre-treated with FcR blocking reagent. At the indicated times, viable cell numbers were enumerated by their ability to exclude the dye trypan blue . Culture supernatant fluid was harvested for IL-6 quantification by ELISA. |
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用途及描述：仅供科研，严禁用于人体(For R&D Only)
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CAS号：242138-07-4
英文名字：Omalizumab;Xolair