Omalizumab

Omalizumab
分子量:149KD
简介:Omalizumab is a recombinant, humanized, monoclonal antibody against human immunoglobulin E (IgE).
产品形式:蛋白原液 。每种装于冻存管中,单抗质量为标示数量1MG或者5MG,体积根据其浓度不同略有不同。

质量标准:
Non-reduced CE-SDS:………………………………………….>90%;
SEC-HPLC:………………………………………………………….>95%;
Bacterial Endotoxins Test:…………………………………. .<10EU/ml
Residual Proteins of Host Cell: ……………………………. <100 ng/mg
Exogenous residual DNA: …………………………………. . <100pg/mg
Residual Protein A: ……………………………………………. .<100 ng/mg
Biological Activity:………………………………………….……. .80% to 125% of standard
储存条件:-70 ± 15℃for long-term storage, 2-8℃for short-term storage, away from light.
运输条件:干冰运输

使用建议:
提供的产品为蛋白原液形式。每种装于冻存管中,单抗质量为标示数量1MG或者5MG,体积根据其浓度不同略有不同。
使用前可以根据自己实验需求进行稀释,溶剂建议用去离子无菌水(货号MA0028,去离子无菌水(细胞培养用水))或者PBS(货号MA0015 PBS(1X),细胞培养级)。
不建议使用含有防腐成分的稀释液进行稀释,会增加细胞或者动物毒性。
稀释前,计算加入多少体积溶剂,请参考购买批次质检单上蛋白浓度,或者询问我司,
举例,质检单显示该批次单抗蛋白原液蛋白浓度为 50mg/ml
对应5MG包装的蛋白原液体积为5mg(质量)/50mg/ml(浓度)=0.1ML,
实验需求蛋白浓度为10mg/ml, 需要稀释倍数为 50/10=5, 则需要加入0.4ML,即400ul PBS或者无菌去离子水即可。
若给药量为固定质量值,比如单次给药0.5MG,对应蛋白原液给药体积为10ul,建议稀释后,提高给药体积降低误差,因为注射器或者移液器吸头残留会影响实验效果。
稀释后的蛋白溶液,可于2-8度冷藏避光保存不超过2周,不建议长期储存。
避免反复冻融,每次冻融蛋白活性会降低约5%左右。
生物活性(仅来自于公开文献,不保证其有效性)

描述
Omalizumab is a recombinant, humanized, monoclonal antibody against human immunoglobulin E (IgE).

靶点

IgE

体外

Tonsillar B cells at a concentration of 1 × 106/mL in supplemented RPMI1640 were treated with 400 U/mL recombinant IL-4 plus 1 μg/mL anti-CD40 in the presence or absence of omalizumab or control antibody (human IgGκ). Omalizumab was reconstituted in sterile PBS at a concentration of 15 mg/mL. Dilutions of omalizumab were made in RPMI1640. In some instances the cells were pre-treated with FcR blocking reagent. At the indicated times, viable cell numbers were enumerated by their ability to exclude the dye trypan blue . Culture supernatant fluid was harvested for IL-6 quantification by ELISA.
Reference:Chan MA, et al.Omalizumab may decrease IgE synthesis by targeting membrane IgE+ human B cells.Clin Transl Allergy. 2013 Sep 2;3(1):29.

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用途及描述:仅供科研,严禁用于人体(For R&D Only)
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CAS号:242138-07-4

英文名字:Omalizumab;Xolair

质量标准:>95%,蛋白原液

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