WSE-7420 EzRIPA Lysis kit,atto代理,atto Extraction kit

WSE-7420 EzRIPA Lysis kit

WSE-7420 EzRIPA Lysis kit,atto代理,atto Extraction kit

目的和应用

全细胞提取准备以下应用
电泳/ Western blotting/免疫沉淀/ ELISA/酶活/等
特性

样品制备电泳、免疫沉淀、ELISA、酶活实验等。
标准组包括蛋白酶抑制剂和磷酸酶抑制剂。
由于EzRIPA裂解试剂盒是基于HEPES的无胺缓冲液,因此制备的样品可以直接进行标记实验。

Purpose and Application

  • Whole cell extract preparation for the following applications
     Electrophoresis/ Western blotting/ Immunoprecipitation/ ELISA/ Enzyme activity/ etc.

Features

  • Sample preparation for electrophoresis, immunoprecipitation, ELISA, enzyme activity experiments, etc.
  • Standard set includes Protease inhibitor and Phosphatase inhibitor.
  • Since EzRIPA Lysis kit is amine-free buffer that based on HEPES buffer, labeling experiments of prepared sample can be applied directly.

Specifications

WSE-7420 EzRIPA Lysis kit
Applicable amount 100 samples, up to 2 x 10⁷ cell/sample
Storage 1 year
Package – EzRIPA Lysis buffer: 100 mL
(20 mM HEPES pH 7.5, 1% IGEPAL® CA-630*, 0.1% SDS, 0.5% Deoxycholic acid, 150 mM Sodium Chloride)
– Protease Inhibitor (100x): 1 mL
(Aprotinin, Pepstatin A, Leupeptin)
– Phosphatase Inhibitor (100X): 10 mL
(Sodium fluoride, Sodium orthovanadate (V), β-sodium glycerophosphate)
* IGEPAL® CA-630 is the same as NonidetP-40, and a trade mark registration of RhonePoulenc AG Co.

Ordering Information

Code No. Description Unit
2332336 WSE-7420 EzRIPA Lysis kit 1 pk